Bioprocess and Biosystems Engineering

, Volume 37, Issue 2, pp 139–149 | Cite as

Inhibition of pathogenic bacterial biofilm by biosurfactant produced by Lysinibacillus fusiformis S9

  • Arun Kumar Pradhan
  • Nilotpala PradhanEmail author
  • Lala Behari Sukla
  • Prasanna Kumar Panda
  • Barda Kanta Mishra
Original Paper


A biosurfactant producing microbe isolated from a river bank was identified as Lysinibacillus fusiformis S9. It was identified with help of biochemical tests and 16S rRNA gene phylogenetic analysis. The biosurfactant S9BS produced was purified and characterized as glycolipid. The biosurfactant showed remarkable inhibition of biofilm formation by pathogenic bacteria like Escherichia coli and Streptococcus mutans. It was interesting to note that at concentration of 40 μg ml−1 the biosurfactant did not show any bactericidal activity but restricted the biofilm formation completely. L. fusiformis is reported for the first time to produce a glycolipid type of biosurfactant capable of inhibiting biofilm formation by pathogenic bacteria. The biosurfactant inhibited bacterial attachment and biofilm formation equally well on hydrophilic as well as hydrophobic surfaces like glass and catheter tubing. This property is significant in many biomedical applications where the molecule should help in preventing biofouling of surfaces without being toxic to biotic system.


Biosurfactants Lysinibacillus fusiformis S9 Glycolipid Biofilm 



The authors like to thank the reviewers for their valuable suggestions in improving the manuscript. A.K.P. would like to express his sincere thanks to Council of Scientific and Industrial Research (CSIR), India for award of Junior Research Fellowship. The authors are thankful to Namrata Mishra for help in phylogenetic tree analysis & Jacintha Esther and Eepsita Priyadarshini for editorial assistance in manuscript preparation.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Arun Kumar Pradhan
    • 1
  • Nilotpala Pradhan
    • 1
    Email author
  • Lala Behari Sukla
    • 1
  • Prasanna Kumar Panda
    • 1
  • Barda Kanta Mishra
    • 1
  1. 1.Bioresources Engineering DepartmentCSIR-Institute of Minerals and Materials TechnologyBhubaneswarIndia

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