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Bioprocess and Biosystems Engineering

, Volume 36, Issue 6, pp 695–703 | Cite as

A new genotype of Miscanthus sacchariflorus Geodae-Uksae 1, identified by growth characteristics and a specific SCAR marker

  • Gi Hong AnEmail author
  • Jung Kon Kim
  • Youn-Ho Moon
  • Young-Lok Cha
  • Young Mi Yoon
  • Bon-Cheol Koo
  • Kwang-Guen Park
Original Paper

Abstract

Miscanthus is referred to as an ideal lignocellulosic bioenergy crop, which can be used to generate heat, power, and fuel, as well as to reduce carbon dioxide emissions. The new Miscanthus sacchariflorus genotype named Geodae-Uksae 1 was recently collected from damp land in southern Korea. This study investigated the growth characteristics of Miscanthus genotypes, and developed a specific, sensitive, and reproducible sequence characterized amplified region (SCAR) marker to distinguish new M. sacchariflorus genotype Geodae-Uksae 1 from other native Miscanthus species in Korea. Growth characteristics such as stem length, stem diameter, and dry weight of Geodae-Uksae 1 were greater than those of normal M. sacchariflorus. The genotypes within Geodae-Uksae 1 were had the highest genetic similarity. A putative 1,800-bp polymorphic sequence specific to Geodae-Uksae 1 was identified with the random amplified polymorphic DNA (RAPD) N8018 primer. The sequence-characterized amplified region (SCAR) primers Geodae 1-F and Geodae 1-R were designed based on the unique RAPD amplicon. The SCAR primers produced a specific 1,799-bp amplicon in authentic Geodae-Uksae 1, whereas no amplification was observed in other Miscanthus species. The SCAR marker could contribute to identify Geodae-Uksae 1 among native Miscanthus species. The new Miscanthus genotype Geodae-Uksae 1 has great potential as an alternative lignocellulosic biomass feedstock for bioenergy productions.

Keywords

Bioenergy Geodae-Uksae 1 Lignocellulosic biomass feedstock Miscanthus RAPD-PCR SCAR marker 

Notes

Acknowledgments

This work was funded by the Government of Korea through the Rural Department Administration (RDA) (No. 12-32-77-PJ007446).

References

  1. 1.
    Atienza SG, Satovid Z, Petersen KK, Dolstra O, Martín A (2002) Preliminary genetic linkage map of Miscanthus sinensis with RAPD markers. Theor Appl Genet 105:946–952CrossRefGoogle Scholar
  2. 2.
    Clifton-Brown JC, Lewandowski I (2002) Screening Miscanthus genotypes in field trials to optimize biomass yield and quality in Southern Germany. Eur J Agron 16:97–110CrossRefGoogle Scholar
  3. 3.
    Christian DG, Riche AB, Yates NE (2008) Growth, yield and mineral content of Miscanthus × giganteus grown as a biofuel for 14 successive harvests. Ind Crop Prod 28:320–327CrossRefGoogle Scholar
  4. 4.
    Przyborowski JA, Sulima P (2010) The analysis of genetic diversity of Salix viminalis genotypes as a potential source of biomass by RAPD markers. Ind Crops Prod 31:395–400CrossRefGoogle Scholar
  5. 5.
    Torney F, Moeller L, Scarpa A, Wang K (2007) Genetic engineering approaches to improve bioethanol production from maize. Curr Opin Biotech 18:193–199CrossRefGoogle Scholar
  6. 6.
    Carpita NC, McCann MM (2008) Maize and sorghum: genetic resources for bioenergy grasses. Trends Plant Sci 13:415–420CrossRefGoogle Scholar
  7. 7.
    Yu J, Zhang X, Tan T (2009) Optimization of media conditions for the production of ethanol from sweet sorghum juice by immobilized Saccharomyces cerevisiae. Biomass Bioenergy 33:521–526CrossRefGoogle Scholar
  8. 8.
    Keshwani DR, Cheng JJ (2008) Switchgrass for bioethanol and other value-added applications: a review. Bioresour Technol 100:1515–1523CrossRefGoogle Scholar
  9. 9.
    Kim JK, An GH, Ahn SH, Moon YH, Cha YL, Bark ST, Choi YH, Suh SJ, Seo SG, Kim SH, Koo BC (2011) Development of SCAR marker for simultaneous identification of Miscanthus sacchariflorus, M. sinensis and M. × giganteus. Bioprocess Biosyst Eng 35:55–59CrossRefGoogle Scholar
  10. 10.
    Greef JM, Deuter M, Jung C, Schondelmaier J (1997) Genetic diversity of European Miscanthus species revealed by AFLP fingerprinting. Genet Resour Crop Evol 44:185–197CrossRefGoogle Scholar
  11. 11.
    Lewandowski I, Clifton-Brown JC, Scurlock JMO, Huisman W (2000) Miscanthus: European experience with a novel energy crop. Biomass Bioenergy 19:209–277CrossRefGoogle Scholar
  12. 12.
    Kaack K, Schwarz KU, Brander PE (2003) Variation in morphology, anatomy and chemistry of stems Miscanthus genotypes differing in mechanical properties. Ind Crops Prod 17:131–142CrossRefGoogle Scholar
  13. 13.
    Jezowski S (2008) Yield traits of six clones of Miscanthus in the first 3 years following planting in Poland. Ind Crops Prod 27:65–68CrossRefGoogle Scholar
  14. 14.
    Atkinson CJ (2009) Establishing perennial grass energy crops in the UK: a review of current propagation options for Miscanthus. Biomass Bioenergy 33:752–759CrossRefGoogle Scholar
  15. 15.
    Chou CH (2009) Miscanthus plants used as an alternative biofuel material: the basic studies on ecology and molecular evolution. Renew Energ 34:1908–1912CrossRefGoogle Scholar
  16. 16.
    Clifton-Brown JC, Stampfl PF, Jones MB (2004) Miscanthus biomass production for energy in Europe and its potential contribution to decreasing fossil fuel carbon emissions. Global Change Biol 10:509–518CrossRefGoogle Scholar
  17. 17.
    Farrell AD, Clifton-Brown JC, Lewandowski IL, Jones MB (2006) Genotypic variation in cold tolerance influences the yield of Miscanthus. Ann Appl Biol 149:337–345CrossRefGoogle Scholar
  18. 18.
    Fernando EM, Maríáa BV, Stephen PL, Germán AB (2008) Meta-analysis of the effects of management factors on Miscanthus × giganteus growth and biomass production. Agric For Meteorol 148:1280–1292CrossRefGoogle Scholar
  19. 19.
    Lewandowski I, Schmidt U (2006) Nitrogen, energy and land efficiencies of Miscanthus, reed canary grass and triticale as determined by the boundary line approach. Agric Ecosyst Environ 112:335–346CrossRefGoogle Scholar
  20. 20.
    Clifton-Brown JC, Lewandowski I (2000) Water use efficiency and biomass partitioning of three different Miscanthus genotypes with limited and unlimited water supply. Ann Bot 86:191–200CrossRefGoogle Scholar
  21. 21.
    Beale CV, Long SP (1995) Can perennial C4 grasses attain high efficiencies of radiant energy conversion in cool climates? Plant Cell Environ 18:641–650CrossRefGoogle Scholar
  22. 22.
    Moon YH, Koo BC, Choi YH, Ahn SH, Bark ST, Cha YL, An GH, Kim JK, Suh SJ (2010) Development of “Miscanthus” the promising bioenergy crop. Korean J Weed Sci 30:330–339 (in Korean)CrossRefGoogle Scholar
  23. 23.
    Welsh J, McClelland M (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucl Acids Res 18:7213–7218CrossRefGoogle Scholar
  24. 24.
    Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP, a new technique for DNA fingerprinting. Nucl Acids Res 23:4407–4414CrossRefGoogle Scholar
  25. 25.
    Folkertsma RT, Roupe van der Voort JNAM, de Groot KE, van Zandvoort PM, Schots A, Gommers FJ, Helder J, Bakker J (1996) Gene pool similarities of potato cyst nematode populations assessed by AFLP analysis. Mol Plant Microbe Interact 9:47–54CrossRefGoogle Scholar
  26. 26.
    Chou CH, Chiang YC, Chiang TY (2000) Genetic variability and phytogeography of Miscanthus sinensis var. condensatus, an apomictic grass, based on RAPD fingerprints. Can J Bot 78:1262–1268Google Scholar
  27. 27.
    Raina SN, Rani V, Kojima T, Ogihara Y, Singh KP, Deyarumath RM (2001) RAPD and ISSR fingerprints as useful genetic markers for analysis of genetic diversity, varietal identification, and phylogenetic relationships in peanut (Arachis hypogaea) cultivars and wild species. Genome 44:763–772Google Scholar
  28. 28.
    Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl Acids Res 18:6531–6535CrossRefGoogle Scholar
  29. 29.
    Fernandez S, Costa AC, Katsuyamza AM, Madeira AMBN, Gruber A (2003) A survey of the inter- and intraspecific RAPD markers of Eimeria spp. of the domestic fowl and the development of reliable diagnostic tools. Parasitol Res 89:437–445Google Scholar
  30. 30.
    Murray YHG, Thompson WF (1980) Rapid isolation of high-molecular-weight plant DNA. Nucl Acids Res 8:4321–4326CrossRefGoogle Scholar
  31. 31.
    Kim SH, Hamada T (2005) Rapid and reliable method of extracting DNA and RNA from sweetpotato, Ipomoea batatas (L.). Lam Biotechnol Lett 27:1841–1845CrossRefGoogle Scholar
  32. 32.
    Jaccard P (1908) Nouvelles recherché sur la distribution florale. Bull Soc Vaud Sci Nat 44:223–270Google Scholar
  33. 33.
    Paran I, Michelmore RW (1993) Development of reliable PCR based markers linked to downy mildew resistance genes in lettuce. Theor Appl Genet 85:985–993CrossRefGoogle Scholar
  34. 34.
    Su H, Wang L, Ge Y, Feng E, Sun J, Liu L (2008) Development of strain-specific SCAR markers for authentication of Ganoderma lucidum. World J Microbiol Biotechnol 24:1223–1226CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Gi Hong An
    • 1
    Email author
  • Jung Kon Kim
    • 2
  • Youn-Ho Moon
    • 1
  • Young-Lok Cha
    • 1
  • Young Mi Yoon
    • 1
  • Bon-Cheol Koo
    • 1
  • Kwang-Guen Park
    • 1
  1. 1.Bioenergy Crop Research CenterNational Institute of Crop Science, Rural Development AdministrationMuanSouth Korea
  2. 2.National Institute of Animal Science, Rural Development AdministrationSuwonSouth Korea

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