Bioprocess and Biosystems Engineering

, Volume 36, Issue 6, pp 695–703 | Cite as

A new genotype of Miscanthus sacchariflorus Geodae-Uksae 1, identified by growth characteristics and a specific SCAR marker

  • Gi Hong AnEmail author
  • Jung Kon Kim
  • Youn-Ho Moon
  • Young-Lok Cha
  • Young Mi Yoon
  • Bon-Cheol Koo
  • Kwang-Guen Park
Original Paper


Miscanthus is referred to as an ideal lignocellulosic bioenergy crop, which can be used to generate heat, power, and fuel, as well as to reduce carbon dioxide emissions. The new Miscanthus sacchariflorus genotype named Geodae-Uksae 1 was recently collected from damp land in southern Korea. This study investigated the growth characteristics of Miscanthus genotypes, and developed a specific, sensitive, and reproducible sequence characterized amplified region (SCAR) marker to distinguish new M. sacchariflorus genotype Geodae-Uksae 1 from other native Miscanthus species in Korea. Growth characteristics such as stem length, stem diameter, and dry weight of Geodae-Uksae 1 were greater than those of normal M. sacchariflorus. The genotypes within Geodae-Uksae 1 were had the highest genetic similarity. A putative 1,800-bp polymorphic sequence specific to Geodae-Uksae 1 was identified with the random amplified polymorphic DNA (RAPD) N8018 primer. The sequence-characterized amplified region (SCAR) primers Geodae 1-F and Geodae 1-R were designed based on the unique RAPD amplicon. The SCAR primers produced a specific 1,799-bp amplicon in authentic Geodae-Uksae 1, whereas no amplification was observed in other Miscanthus species. The SCAR marker could contribute to identify Geodae-Uksae 1 among native Miscanthus species. The new Miscanthus genotype Geodae-Uksae 1 has great potential as an alternative lignocellulosic biomass feedstock for bioenergy productions.


Bioenergy Geodae-Uksae 1 Lignocellulosic biomass feedstock Miscanthus RAPD-PCR SCAR marker 



This work was funded by the Government of Korea through the Rural Department Administration (RDA) (No. 12-32-77-PJ007446).


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Gi Hong An
    • 1
    Email author
  • Jung Kon Kim
    • 2
  • Youn-Ho Moon
    • 1
  • Young-Lok Cha
    • 1
  • Young Mi Yoon
    • 1
  • Bon-Cheol Koo
    • 1
  • Kwang-Guen Park
    • 1
  1. 1.Bioenergy Crop Research CenterNational Institute of Crop Science, Rural Development AdministrationMuanSouth Korea
  2. 2.National Institute of Animal Science, Rural Development AdministrationSuwonSouth Korea

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