Bioprocess and Biosystems Engineering

, Volume 36, Issue 10, pp 1463–1474

Enhancing polyhydroxybutyrate production from high cell density fed-batch fermentation of Bacillus megaterium BA-019

  • Pawut Kanjanachumpol
  • Songsri Kulpreecha
  • Vasana Tolieng
  • Nuttha Thongchul
Original Paper

DOI: 10.1007/s00449-013-0885-7

Cite this article as:
Kanjanachumpol, P., Kulpreecha, S., Tolieng, V. et al. Bioprocess Biosyst Eng (2013) 36: 1463. doi:10.1007/s00449-013-0885-7

Abstract

This study demonstrated the improved polyhydroxybutyrate (PHB) production via high cell density cultivation of Bacillus megaterium BA-019 with balanced initial total sugar concentration and carbon to nitrogen (C/N) weight ratio. In the 10 L stirred fermentor operated at 30 °C, pH 7.0, 600 rpm, and 1.0 vvm air, with the initial total sugar concentration of 60 g/L and urea at the C/N weight ratio of 10:1, 32.48 g/L cell biomass with the corresponding PHB weight content of 26.94 % and volumetric productivity of 0.73 g/L h were obtained from batch cultivation. Continuing cultivation by intermittent feeding of the sugarcane molasses along with urea at the C/N weight ratio of 12.5:1 gave much improved biomass and PHB production (90.71 g/L biomass with 45.84 % PHB content and 1.73 g/L h PHB productivity). Similar biomass and PHB yields were obtained in the 90 L stirred fermentor when using the impeller tip speed as the scale-up criterion.

Keywords

Polyhydroxybutyrate High cell density production Fed-batch cultivation Fermentation scale-up Sugarcane molasses Bacillus megaterium 

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Pawut Kanjanachumpol
    • 1
  • Songsri Kulpreecha
    • 1
  • Vasana Tolieng
    • 2
  • Nuttha Thongchul
    • 2
  1. 1.Department of Microbiology, Faculty of ScienceChulalongkorn UniversityBangkokThailand
  2. 2.Institute of Biotechnology and Genetic EngineeringChulalongkorn UniversityBangkokThailand

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