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Bioprocess and Biosystems Engineering

, Volume 36, Issue 9, pp 1229–1233 | Cite as

Microbial transformation and sorption of anthracene in liquid culture

  • Tony HadibarataEmail author
  • Meor Mohd Fikri Ahmad Zubir
  • Rubiyatno
  • Teh Zee Chuang
Original Paper

Abstract

Armillaria sp. F022, a white-rot fungus isolated from decayed wood in tropical rain forest was used to biodegrade anthracene in cultured medium. The percentage of anthracene removal by Armillaria sp. F022 reached 13 % after 7 days and at the end of the experiment, anthracene removal level was at 87 %. The anthracene removal through sorption and transformation was investigated. 69 % of eliminated anthracene was transformed by Armillaria sp. F022 to form other organic structure, while only 18 % was absorbed in the mycelia. In the kinetic experiment, anthracene dissipation will not stop even though the biomass had stopped growing. Anthracene removal by Armillaria sp. F022 was correlated with protein concentration (whole biomass) in the culture. The production of enzyme was affected by biomass production. Anthracene was transformed to two stable metabolic products. The metabolites were extracted in ethyl-acetate, isolated by column chromatography, and then identified using gas chromatography–mass spectrometry (GC–MS).

Keywords

Anthracene degradation Biotransformation Coumarin and dihydroxynaphthalene Dissipation potential Sorption 

Notes

Acknowledgments

A part of this project was financially supported by Universiti Teknologi Malaysia (RUG Vote QJ1.3000.2522.02H65).

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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Tony Hadibarata
    • 1
    Email author
  • Meor Mohd Fikri Ahmad Zubir
    • 1
  • Rubiyatno
    • 1
  • Teh Zee Chuang
    • 1
  1. 1.Institute of Environmental and Water Resources Management, Faculty of Civil EngineeringUniversiti Teknologi MalaysiaSkudaiMalaysia

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