Bioprocess and Biosystems Engineering

, Volume 36, Issue 5, pp 579–589 | Cite as

The effect of biological sulfate reduction on anaerobic color removal in anaerobic–aerobic sequencing batch reactors

  • Kevser Cirik
  • Mehmet Kitis
  • Ozer CinarEmail author
Original Paper


Combination of anaerobic–aerobic sequencing processes result in both anaerobic color removal and aerobic aromatic amine removal during the treatment of dye-containing wastewaters. The aim of the present study was to gain more insight into the competitive biochemical reactions between sulfate and azo dye in the presence of glucose as electron donor source. For this aim, anaerobic–aerobic sequencing batch reactor fed with a simulated textile effluent including Remazol Brilliant Violet 5R (RBV 5R) azo dye was operated with a total cycle time of 12 h including anaerobic (6 h) and aerobic cycles (6 h). Microorganism grown under anaerobic phase of the reactor was exposed to different amounts of competitive electron acceptor (sulfate). Performance of the anaerobic phase was determined by monitoring color removal efficiency, oxidation reduction potential, color removal rate, chemical oxygen demand (COD), color, specific anaerobic enzyme (azo reductase) and aerobic enzyme (catechol 1,2-dioxygenase), and formation of aromatic amines. The presence of sulfate was not found to significantly affect dye decolorization. Sulfate and azo dye reductions took place simultaneously in all operational conditions and increase in the sulfate concentration generally stimulated the reduction of RBV 5R. However, sulfate accumulation under anaerobic conditions was observed proportional to increasing sulfate concentration.


Anaerobic color removal Sulfate Remazol Brilliant Violet-5R Sequencing batch reactor 


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  1. 1.Department of Environmental EngineeringKahramanmaraş Sütçü İmam UniversityKahramanmarasTurkey
  2. 2.Department of Environmental EngineeringSüleyman Demirel UniversityIspartaTurkey
  3. 3.Bioengineering and Genetics ProgramInternational University of SarajevoSarajevoBosnia and Herzegovina

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