Bioprocess and Biosystems Engineering

, Volume 35, Issue 7, pp 1201–1208 | Cite as

Impact of nitrogen concentration on validamycin A production and related gene transcription in fermentation of Streptomyces hygroscopicus 5008

  • Zhen-Hua Wei
  • Linquan Bai
  • Zixin Deng
  • Jian-Jiang ZhongEmail author
Original Paper


Validamycin A (VAL-A) is an important and widely used agricultural antibiotic. In this study, statistical screening designs were applied to identify significant medium variables for VAL-A production and to find their optimal levels. The optimized medium caused 70% enhancement of VAL-A production. The difference between optimized medium and original medium suggested that low nitrogen source level might attribute to the enhancement of VAL-A production. The addition of different nitrogen sources to the optimized medium inhibited VAL-A production, which confirmed the importance of nitrogen concentration for VAL-A production. Furthermore, differences in structural gene transcription and enzyme activity between the two media were assayed. The results showed that lower nitrogen level in the optimized medium could regulate VAL-A production in gene transcriptional level. Our previous study indicated that the transcription of VAL-A structural genes could be enhanced at elevated temperature. In this work, the increased fermentation temperature from 37 to 42 °C with the optimized medium enhanced VAL-A production by 39%, which testified to the importance of structural gene transcription in VAL-A production. The information is useful for further VAL-A production enhancement.


Validamycin A Gene transcription Secondary metabolites Nitrogen source 



We appreciate the financial support from the National 863 High Technology Program (Project # 2006AA10A202) of the MOST and the National Natural Science Foundation of China (NSFC project No. 30821005). JJZ also appreciates the National 985 Project and the University Distinguished Professorship program (SJTU).

Supplementary material

449_2012_707_MOESM1_ESM.doc (142 kb)
Supplementary material 1 (DOC 142 kb)


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Zhen-Hua Wei
    • 1
  • Linquan Bai
    • 2
  • Zixin Deng
    • 2
  • Jian-Jiang Zhong
    • 1
    • 2
    Email author
  1. 1.State Key Laboratory of Bioreactor Engineering, School of BiotechnologyEast China University of Science and TechnologyShanghaiChina
  2. 2.State Key Laboratory of Microbial Metabolism, School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghaiChina

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