Bioprocess and Biosystems Engineering

, Volume 35, Issue 7, pp 1193–1200 | Cite as

Chitin and L(+)-lactic acid production from crab (Callinectes bellicosus) wastes by fermentation of Lactobacillus sp. B2 using sugar cane molasses as carbon source

  • Belem Flores-Albino
  • Ladislao Arias
  • Jorge Gómez
  • Alberto Castillo
  • Miquel Gimeno
  • Keiko ShiraiEmail author
Original Paper


Crab wastes are employed for simultaneous production of chitin and L(+)-lactic acid by submerged fermentation of Lactobacillus sp. B2 using sugar cane molasses as carbon source. Response surface methodology was applied to design the culture media considering demineralization. Fermentations in stirred tank reactor (2L) using selected conditions produced 88% demineralization and 56% deproteinization with 34% yield of chitin and 19.5 gL−1 of lactic acid (77% yield). The chitin purified from fermentation displayed 95% degree of acetylation and 0.81 and 1 ± 0.125% of residual ash and protein contents, respectively.


Chitin Lactic acid Lactic acid bacteria Crab wastes Sugar cane Molasses 



We would like to thank to Institute of Science and Technology of Mexico City (ICyTDF No. PICSA11-69) for funding and to CONACYT (Government of Mexico) for scholarship (BFA).

Supplementary material

449_2012_706_MOESM1_ESM.doc (140 kb)
Supplementary material 1 (DOC 140 kb)


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Belem Flores-Albino
    • 1
  • Ladislao Arias
    • 1
  • Jorge Gómez
    • 1
  • Alberto Castillo
    • 2
  • Miquel Gimeno
    • 3
  • Keiko Shirai
    • 1
    Email author
  1. 1.Laboratory of Biopolymers, Department of BiotechnologyUniversidad Autonoma MetropolitanaMexico CityMexico
  2. 2.Department of MathematicsUniversidad Autonoma MetropolitanaMexico CityMexico
  3. 3.Depto. Alimentos y biotecnología, Facultad de QuímicaUniversidad Nacional Autónoma de MéxicoMéxico DFMexico

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