Bioprocess and Biosystems Engineering

, Volume 35, Issue 6, pp 963–975 | Cite as

Use of focussed beam reflectance measurement (FBRM) for monitoring changes in biomass concentration

  • Jessica Whelan
  • Eilis Murphy
  • Alan Pearson
  • Paul Jeffers
  • Patricia Kieran
  • Susan McDonnell
  • Brian Glennon
Original Paper

Abstract

The potential of focussed beam reflectance measurement (FBRM) as a tool to monitor changes in biomass concentration was investigated in a number of biological systems. The measurement technique was applied to two morphologically dissimilar plant cell suspension cultures, Morinda citrifolia and Centaurea calcitrapa, to a filamentous bacteria, Streptomyces natalensis, to high density cultures of Escherichia coli and to a murine Sp2/0 hybridoma suspension cell line, 3–2.19. In all cases, the biomass concentration proved to be correlated with total FBRM counts. The nature of the correlation varied between systems and was influenced by the concentration, nature, size and morphology of the particle under investigation.

Keywords

Biomass concentration On-line monitoring FBRM Cell morphology 

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Jessica Whelan
    • 1
  • Eilis Murphy
    • 2
  • Alan Pearson
    • 3
  • Paul Jeffers
    • 4
  • Patricia Kieran
    • 1
  • Susan McDonnell
    • 1
  • Brian Glennon
    • 1
  1. 1.UCD School of Chemical and Bioprocess EngineeringUCDDublin 4Ireland
  2. 2.Training DepartmentNational Institute of Bioprocessing Research and TrainingDublin 4Ireland
  3. 3.Pharmaceutical Operations, MSD IrelandTipperaryIreland
  4. 4.Process Analytical Sciences GroupPfizer Ireland PharmaceuticalsCorkIreland

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