Bioprocess and Biosystems Engineering

, Volume 35, Issue 5, pp 721–728 | Cite as

Heterotrophic nitrification by Achromobacter xylosoxidans S18 isolated from a small-scale slaughterhouse wastewater

  • Pradyut Kundu
  • Arnab Pramanik
  • Sayani Mitra
  • Jayanta Debabrata Choudhury
  • Joydeep Mukherjee
  • Somnath Mukherjee
Original Paper


Heterotrophic carbon utilizing microbes were acclimatized in the laboratory by inoculating sludge collected from the waste discharge pond of a small-scale rural abattoir in India in a nutrient solution intermittently fed with glucose and ammonium chloride. Cultures of 10 well-developed isolates were selected and grown in a basal medium containing glucose and ammonium chloride. Culture supernatants were periodically analyzed for ammonium nitrogen (NH4+-N) and chemical oxygen demand (COD). Polyphasic taxonomic study of the most active nitrifier (S18) was done. Half saturation concentration (Ks), maximum rate of substrate utilization (k), yield coefficient (Y) and decay coefficient (Kd) were determined from the Lineweaver–Burk plot using the modified Monod equation. S18 was able to remove 97 ± 2% of (NH4+-N) and 88 ± 3% of COD. Molecular phylogenetic study supported by physiological and biochemical characteristics assigned S18 as Achromobacter xylosoxidans. Nitrification activity of A. xylosoxidans was demonstrated for the first time, while interestingly, the distinctive anaerobic denitrification property was preserved in S18. Ks values were determined as 232.13 ± 1.5 mg/l for COD reduction and 2.131 ± 1.9 mg/l for NH4+-N utilization. Yield coefficients obtained were 0.4423 ± 0.1134 mg of MLVSS/mg of COD and 0.2461 ± 0.0793 mg of MLVSS/mg of NH4+-N while the decay coefficients were 0.0627 ± 0.0013 per day and 0.0514 ± 0.0008 per day, respectively. After a contact period of 24 h, 650 ± 5 mg/l solids were produced when the initial concentration of COD and NH4+-N were 1820 ± 10 mg/l and 120 ± 5.5 mg/l, respectively. This is the first report on the kinetic coefficients for carbon oxidation and nitrification by a single bacterium isolated from slaughterhouse wastewater.


Nitrification Achromobacter Slaughterhouse Wastewater Kinetic constants 

Supplementary material

449_2011_652_MOESM1_ESM.doc (36 kb)
Supplementary material 1 (DOC 36 kb)


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Pradyut Kundu
    • 1
  • Arnab Pramanik
    • 2
  • Sayani Mitra
    • 2
  • Jayanta Debabrata Choudhury
    • 2
  • Joydeep Mukherjee
    • 2
  • Somnath Mukherjee
    • 1
  1. 1.Department of Civil EngineeringJadavpur UniversityKolkataIndia
  2. 2.School of Environmental StudiesJadavpur UniversityKolkataIndia

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