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Bioprocess and Biosystems Engineering

, Volume 35, Issue 1–2, pp 235–240 | Cite as

Effect of ionic liquids on enzymatic synthesis of caffeic acid phenethyl ester

  • Sung Ho Ha
  • Tran Van Anh
  • Sang Hyun Lee
  • Yoon-Mo Koo
Original Paper

Abstract

Although caffeic acid phenethyl ester (CAPE), an active flavonoid, plays an important role in the antioxidant activity of honeybee propolis, the isolation of CAPE from honeybee propolis is time-consuming due to wide variety of impurities present. Therefore, biochemical method to synthesize CAPE was investigated in this study. Since ionic liquids (ILs) possess some unique characteristics as appreciated alternatives to conventional solvents for certain biotransformation, the effect of ILs as reaction media for enzymatic synthesis of CAPE was assessed. Several factors including substrate molar ratio, and reaction temperature affecting the conversion yield of lipase-catalyzed CAPE synthesis were also investigated. Reaction yields were significantly higher in hydrophobic ILs than in hydrophilic ILs (almost zero). Among nine hydrophobic ILs tested, the highest conversion of synthetic reaction was obtained in 1-ethyl-3-methylimidazolium bis[(trifluoromethyl)sulfonyl]imide ([Emim][Tf2N]). A reaction temperature of 70 °C was found to give high conversion. In addition, optimal substrate molar ratio between phenethyl alcohol and caffeic acid (CA) was decreased significantly from 92:1 to 30:1 when ILs were used instead of isooctane.

Keywords

Ionic liquids Caffeic acid phenethyl ester Lipase Esterification 

Notes

Acknowledgments

This paper has been supported by 2011 Hannam University Research Fund.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.Department of Chemical Engineering and Nano-Bio TechnologyHannam UniversityDaejeonKorea
  2. 2.Department of Biological EngineeringInha UniversityIncheonKorea
  3. 3.Department of Microbial EngineeringKonkuk UniversitySeoulKorea

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