Bioprocess and Biosystems Engineering

, Volume 35, Issue 1–2, pp 247–254 | Cite as

A newly isolated Streptomyces sp. CS392 producing three antimicrobial compounds

  • Seung Sik Cho
  • Yun Hee Choi
  • Jaya Ram Simkhada
  • Poonam Mander
  • Da Jeong Park
  • Jin Cheol Yoo
Original Paper
First Online:
Received:
Accepted:

Abstract

With the aim of isolating new microbes capable of producing strong antimicrobial substances, strain CS392 was screened from 700 soil isolates preserved in our laboratory. The strain was related to genus Streptomyces based on various characteristics. Three highly active antimicrobial compounds, C1, C2 and C3, produced by the strain were purified by solvent extraction followed by silica gel column chromatography. These compounds were highly active against various Gram-positive resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Staphylococcus aureus (VRSA), and vancomycin-resistant Enterococcus (VRE). Among three, C3 was the most active against MRSA and VRSA with minimal inhibitory concentration (MIC) of 2 μg/ml while C2 and C3 had MIC values of 4 μg/ml for the strains. In case of Bacillus subtilis ATCC6633, C1 and C3 were more effective with MIC values of 0.5 μg/ml than C2 with MIC of 2 μg/ml. Those antibiotics were variably active (MIC of 4–32 μg/ml) against Micrococcus luteus ATCC 9341, Enterococcus faecalis ATCC 29212, Mycobacterium smegmatis ATCC 9341 and VRE.

Keywords

Antimicrobial compounds Purification Streptomyces sp. CS392 
This is a preview of subscription content, log in to check access.

Notes

Acknowledgments

This work was supported by the Korea Research Foundation (KRF) grant funded by the Korean government (MEST) (2010-0029178) and Technology Development Program for Agriculture and Forestry, Ministry of Agriculture and Forestry, republic of Korea, 2007 (307014-5).

References

  1. 1.
    Osada H (1998) Actinomycetes, How Fascinating Microorganisms. Actinomycetologica 12:85–88CrossRefGoogle Scholar
  2. 2.
    Saadoun I, Gharaibeh R (2003) The Streptomyces flora of Badia region of Jordan and its potential as a source of antibiotics active against antibiotic-resistant bacteria. J Arid Environ 53:365–371CrossRefGoogle Scholar
  3. 3.
    Watve MG, Tickoo R, Jog MM, Bhole BD (2001) How many antibiotics are produced by the genus Streptomyces. Arch Microbiol 176:386–390CrossRefGoogle Scholar
  4. 4.
    Miyadoh S (1993) Research on antibiotic screening in Japan over the last decade: a producing microorganisms approach. Actinomycetologica 9:100–106CrossRefGoogle Scholar
  5. 5.
    Ferber D (2002) Antibiotic resistance. Livestock feed ban preserves drugs’ power. Science 295:27–28CrossRefGoogle Scholar
  6. 6.
    Goossens H, Ferech M, Vander Stichele R, Elseviers M (2005) Outpatient antibiotic use in Europe and association with resistance: a cross-national database study. Lancet 365:579–587Google Scholar
  7. 7.
    Mathew AG, Cissell R, Liamthong S (2007) Antibiotic resistance in bacteria associated with food animals: a United States perspective of livestock production. Foodborne Pathog Dis 4:115–133CrossRefGoogle Scholar
  8. 8.
    Joakim Larsson DG, Fick J (2009) Transparency throughout the production chain––a way to reduce pollution from the manufacturing of pharmaceuticals? Regul Toxicol Pharmacol 53:161–163CrossRefGoogle Scholar
  9. 9.
    Klevens RM, Edwards JR, Tenover FC, McDonald LC, Horan T et al (2006) Changes in the epidemiology of methicillin-resistant Staphylococcus aureus in intensive care units in US hospitals, 1992–2003. Clin Infect Dis 42:389–391CrossRefGoogle Scholar
  10. 10.
    Moran GJ, Krishnadasan A, Gorwitz RJ, Fosheim GE, McDougal LK et al (2006) Methicillin-resistant S. aureus infections among patients in the emergency department. N Engl J Med 355:666–674CrossRefGoogle Scholar
  11. 11.
    Rotun SS, McMath V, Schoonmaker DJ, Maupin PS, Tenover FC, Hill BC, Ackman DM (1999) Staphylococcus aureus with reduced susceptibility to vancomycin isolated from a patient with fatal bacteremia. Emerg Infect Dis 5:147–149CrossRefGoogle Scholar
  12. 12.
    Smith TL, Pearson ML, Wilcox KR, Cruz C, Lancaster MV, Robinson-Dunn B, Tenover FC, Zervos MJ, Band JD, White E, Jarvis WR (1999) Emergence of vancomycin resistance in Staphylococcus aureus. N Engl J Med 340:493–501CrossRefGoogle Scholar
  13. 13.
    Tsiodras S, Gold HS, Sakoulas G, Eliopoulos GM, Wennersten C, Venkataraman L et al (2001) Linezolid resistance in a clinical isolate of Staphylococcus aureus. Lancet 358:207–208CrossRefGoogle Scholar
  14. 14.
    Wisplinghoff H, Bischoff T, Tallent SM, Seifert H, Wenzel RP et al (2004) Nosocomial bloodstream infections in US hospitals: analysis of 24, 179 cases from a prospective nationwide surveillance study. Clin Infect Dis 39:309–317CrossRefGoogle Scholar
  15. 15.
    Lechevalier HA, Williams ST, Sharpe ME, Holt JG (eds) (1989) The Actinomycetes: a practical guide to genetic identification of Actinomycetes Bergey’s manual of systematic bacteriology. Williams and Wilkins, Sydney, pp 2344–2347Google Scholar
  16. 16.
    Sohng JK, Yamaguchi T, Seong CN, Baik KS, Park SC et al (2008) Production, isolation and biological activity of nargenicin from Nocardia sp. CS682. Arch Pharm Res 31:1339–1345CrossRefGoogle Scholar
  17. 17.
    Yoo JC, Kim JH, Ha JW, Park NS, Sohng JK et al (2007) Production and biological activity of laidlomycin, anti-MRSA/VRE antibiotic from Streptomyces sp. CS684. J Microbiol 45:6–10Google Scholar
  18. 18.
    Shirling EB, Gottlieb D (1966) Methods for characterization of streptomycetes species. Int J System Bacteriol 16:313–340CrossRefGoogle Scholar
  19. 19.
    Kenneth LK, Deanne BJ (1995) Color universal language and dictionary of names. United States Department of Commerce National Bureau of standards. Washington, DC, 20234Google Scholar
  20. 20.
    Pridham TG, Gottlieb GD (1948) The utilization of carbon compounds by some Actinomycetales as an aid for species determination. J Bacteriol 56:107–114Google Scholar
  21. 21.
    Pridham TG, Anderson P, Foley C, Lindenfelser LA, Hesseltine CW et al (1956) A selection of media for maintenance and taxonomic study of Streptomyces. Antibiot Annu 56:947–953Google Scholar
  22. 22.
    Dye DW (1962) The inadequacy of the usual determinative tests for the identification of Xanthomonas spp. NZ J Sci 5:393–416Google Scholar
  23. 23.
    Chapman GS (1952) A simple method for making multiple tests of a microorganism. J Bacteriol 63:147Google Scholar
  24. 24.
    Cowan ST (1974) Cowan & Steel’s manual for the identification of medical bacteria. Cambridge University Press, New YorkGoogle Scholar
  25. 25.
    Elwan SH, MRE-NaMSA (1977) Characteristics of lipases in the growth filtrate dialysate of Bacillus stearothermophilus grown at 55 degrees Celsius using a tributyrin cup-plate assay. Bull Fac Sci, Riyadh Univ 8:105–119Google Scholar
  26. 26.
    Edwards U, Rogall T, Blocker H, Emde M, Bottger EC (1989) Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res 17:7843–7853CrossRefGoogle Scholar
  27. 27.
    Mellouli L, Ben Ameur-Mehdi R, Sioud S, Salem M, Bejar S (2003) Isolation, purification and partial characterization of antibacterial activities produced by a newly isolated Streptomyces sp. US24 strain. Res Microbiol 154:345–352CrossRefGoogle Scholar
  28. 28.
    Labeda DP, Lechevalier MP, Testa RT (1997) Streptomyces stramineus sp. nov., a new species of the verticillate streptomycetes. Int J Syst Bacteriol 47:747–753CrossRefGoogle Scholar
  29. 29.
    Chin JN, Rybak MJ, Cheung CM, Savage PB (2007) Antimicrobial activities of ceragenins against clinical isolates of resistant Staphylococcus aureus. Antimicrob Agents Chemother 51:1268–1273CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Seung Sik Cho
    • 1
  • Yun Hee Choi
    • 1
  • Jaya Ram Simkhada
    • 1
  • Poonam Mander
    • 1
  • Da Jeong Park
    • 1
  • Jin Cheol Yoo
    • 1
  1. 1.Department of Pharmacy, College of PharmacyChosun UniversityGwangjuKorea

Personalised recommendations