Production of nattokinase by high cell density fed-batch culture of Bacillus subtilis
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Bacillus subtilis was cultivated to high cell density for nattokinase production by pH-stat fed-batch culture. A concentrated mixture solution of glucose and peptone was automatically added by acid-supplying pump when culture pH rose above high limit. Effect of the ratio of glucose to peptone in feeding solution was investigated on cell growth and nattokinase production by changing the ratio from 0.2 to 5 g glucose/g peptone. The highest cell concentration was 77 g/L when the ratio was 0.2 g glucose/g peptone. Cell concentration decreased with increasing the ratio of glucose to peptone in feeding solution, while the optimum condition existed for nattokinase production. The highest nattokinase activity was 14,500 unit/mL at a ratio of 0.33 g glucose/g peptone, which was 4.3 times higher than that in batch culture.
KeywordsNattokinase Bacillus subtilis Fed-batch culture High cell density culture
- 2.Kim W, Choi K, Kim Y, Park H, Choi J, Lee Y, Oh H, Kwon I, Lee S (1996) Purification and characterization of a fibrinolytic enzyme produced from Bacillus sp. strain CK 11-4 screened from Chungkook-Jang. Appl Environ Microbiol 62:2482–2488Google Scholar
- 6.Kim BS (2007) High cell density culture techniques for production of industrial products. In: Shaw J-F, Hou CT (eds) Biocatalysis and biotechnology for functional foods and industrial products. CRC Press, Boca Raton, FL, pp 505–520Google Scholar
- 16.Po TC, Chiang C-J, Chao Y-P (2007) Strategy to approach stable production of recombinant nattokinase in Bacillus subtilis. Biotechnol Prog 23:808–813Google Scholar