Bioprocess and Biosystems Engineering

, Volume 34, Issue 1, pp 113–119 | Cite as

Heterologous expression of an alginate lyase from Streptomyces sp. ALG-5 in Escherichia coli and its use for preparation of the magnetic nanoparticle-immobilized enzymes

  • Jung Won Shin
  • Sung Hee Choi
  • Dong Eun Kim
  • Hee Sook Kim
  • Jae-Hwa Lee
  • In Su Lee
  • Eun Yeol Lee
Original Paper


The marine alginate lyase from Streptomyces sp. ALG-5, which specifically degrades poly-G block of alginate, was functionally expressed as a His-tagged form with an Escherichia coli expression system. The recombinant alginate lyase expressed with pColdI at 15 °C exhibited the highest alginate-degrading activity. The recombinant alginate lyase was efficiently immobilized onto two types of magnetic nanoparticles, superparamagnetic iron oxide nanoparticle, and hybrid magnetic silica nanoparticle, based on the affinity between His-tag and Ni2+ that displayed on the surfaces of nanoparticles. An alginate oligosaccharide mixture consisting of dimer and trimer was prepared by the immobilized alginate lyase. The immobilized enzymes were re-used repeatedly more than 10 times after magnetic separation.


Immobilization Alginate lyase Alginate oligosaccharide Streptomyces sp. ALG-5 



This work was supported by New & Renewable Energy R&D program (20093020090020) under the Korea Ministry of Knowledge Economy (MKE).


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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Jung Won Shin
    • 1
  • Sung Hee Choi
    • 2
  • Dong Eun Kim
    • 2
  • Hee Sook Kim
    • 2
  • Jae-Hwa Lee
    • 3
  • In Su Lee
    • 4
  • Eun Yeol Lee
    • 1
  1. 1.Department of Chemical Engineering, Industrial Liaison Research CenterKyung Hee UniversityGyeonggi-doRepublic of Korea
  2. 2.Department of Food Science and BiotechnologyKyungsung UniversityPusanRepublic of Korea
  3. 3.Department of Bioscience and BiotechnologySilla UniversityPusanRepublic of Korea
  4. 4.Department of Applied ChemistryKyung Hee UniversityGyeonggi-doRepublic of Korea

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