Bioprocess and Biosystems Engineering

, Volume 34, Issue 1, pp 113–119 | Cite as

Heterologous expression of an alginate lyase from Streptomyces sp. ALG-5 in Escherichia coli and its use for preparation of the magnetic nanoparticle-immobilized enzymes

  • Jung Won Shin
  • Sung Hee Choi
  • Dong Eun Kim
  • Hee Sook Kim
  • Jae-Hwa Lee
  • In Su Lee
  • Eun Yeol Lee
Original Paper

Abstract

The marine alginate lyase from Streptomyces sp. ALG-5, which specifically degrades poly-G block of alginate, was functionally expressed as a His-tagged form with an Escherichia coli expression system. The recombinant alginate lyase expressed with pColdI at 15 °C exhibited the highest alginate-degrading activity. The recombinant alginate lyase was efficiently immobilized onto two types of magnetic nanoparticles, superparamagnetic iron oxide nanoparticle, and hybrid magnetic silica nanoparticle, based on the affinity between His-tag and Ni2+ that displayed on the surfaces of nanoparticles. An alginate oligosaccharide mixture consisting of dimer and trimer was prepared by the immobilized alginate lyase. The immobilized enzymes were re-used repeatedly more than 10 times after magnetic separation.

Keywords

Immobilization Alginate lyase Alginate oligosaccharide Streptomyces sp. ALG-5 

References

  1. 1.
    Wong TY, Preston LA, Schiller NL (2000) Alginate lyase: review of major sources and enzyme characteristics, structure-function analysis, biological roles, and application. Annu Rev Microbiol 54:289–340CrossRefGoogle Scholar
  2. 2.
    Kim DE, Lee EY, Kim HS (2009) Cloning and characterization of alginate lyase from a marine bacterium Streptomyces sp. ALG-5. Mar Biotechnol 11:10–16CrossRefGoogle Scholar
  3. 3.
    Zhang Z, Yu G, Guan H, Zhao X, Du Y, Jiang X (2004) Preparation and structure elucidation of alginate oligosaccharides degraded by alginate lyase from Vibrio sp. 510. Carbohydr Res 258:187–197Google Scholar
  4. 4.
    Kawamoto H, Horibe A, Miki Y, Kimura T, Tanaka K, Nakagawa T, Kawamukai M, Matsuda H (2006) Cloning and sequencing analysis of alginate lyase genes from the marine bacterium Vibrio sp. O2. Mar Biotechnol 8:481–490CrossRefGoogle Scholar
  5. 5.
    Matsubara Y, Kawada R, Iwasaki K, Kimura Y, Oda T, Muramatsu T (2000) Cloning and sequence analysis of a gene (aly PG) encoding poly(α-l-guluronate) lyase from Corynebacterium sp. strain ALY-1. J Biosci Bioeng 89:199–202CrossRefGoogle Scholar
  6. 6.
    Osawa T, Matsubara Y, Muramatsu T, Kimura M, Kakuta Y (2005) Crystal structure of the alginate (poly-α-l-guluronate) lyase from Corynebacterium sp. at 1.2 Å resolution. J Mol Biol 345:1111–1118CrossRefGoogle Scholar
  7. 7.
    Iwamoto M, Kurachi M, Nakashima T, Kim D, Yamaguch K, Oda T, Iwamoto Y, Muramatsu T (2005) Structure–activity relationship of alginate oligosaccharides in the induction of cytokine production from RAW264.7 cells. FEBS Lett 579:4423–4429CrossRefGoogle Scholar
  8. 8.
    Kawada A, Hiura N, Tajima S, Takahara H (1999) Alginate oligosaccharides stimulate VEGF-mediated growth and migration of human endothelial cells. Arch Dermatol Res 291:542–547CrossRefGoogle Scholar
  9. 9.
    Cao L, Xie L, Xue X, Tan H, Liu Y, Zhou S (2007) Purification and characterization of alginate lyase from Streptomyces species strain A5 isolated from Banana Rhizosphere. J Agric Food Chem 55:5113–5117CrossRefGoogle Scholar
  10. 10.
    Gimmestad M, Ertesvåg H, Heggeset TMB, Aarstad O, Svanem BIG, Valla S (2009) Characterization of three new Azotobacter vinelandii alginate lyases, one of which is involved in cyst germination. J Bacteriol 191:4845–4853CrossRefGoogle Scholar
  11. 11.
    Alkawash MA, Soothill JS, Schiller NL (2006) Alginate lyase enhances antibiotic killing of mucoid Pseudomonas aeruginosa in biofilms. APMIS 114:131–138CrossRefGoogle Scholar
  12. 12.
    Chisti Y (2008) Biodiesel from microalgae beats bioethanol. Trends Biotechnol 26:126–131CrossRefGoogle Scholar
  13. 13.
    Beer LL, Boyd ES, Peters JW, Posewitz MC (2009) Engineering algae for biohydrogen and biofuel production. Curr Opin Biotechnol 20:264–271CrossRefGoogle Scholar
  14. 14.
    Vasudevan PT, Briggs M (2008) Biodiesel production-current state of the art and challenges. J Ind Microbiol Biotechnol 35:421–430CrossRefGoogle Scholar
  15. 15.
    Choi D, Ryu B-Y, Piao YL, Choi S-K, Jo B-W, Shin W-S, Cho H (2008) Studies on saccharification from alginate using Stenotrophomonas maltophilia. J Ind Eng Chem 14:182–186Google Scholar
  16. 16.
    Lee IS, Lee N, Park J, Kim BH, Yi YW, Kim T, Kim TK, Lee IH, Paik SR, Hyeon T (2006) Ni/NiO core/shell nanoparticles for selective binding and magnetic separation of histidine. J Am Chem Soc 128:10658–10659CrossRefGoogle Scholar
  17. 17.
    Lee KS, Lee IS (2008) Decoration of superparamagnetic iron oxide nanoparticles with Ni2+: agent to bind and separate histidine-tagged proteins. Chem Commun 2008:709–711CrossRefGoogle Scholar
  18. 18.
    Lee KS, Woo MH, Kim HS, Lee EY, Lee IS (2009) Synthesis of hybrid Fe3O4/silica/NiO superstructures and their application as magnetically separable high-performance biocatalysts. Chem Commun 25:3780–3782CrossRefGoogle Scholar
  19. 19.
    Yoon H-J, Hashimoto W, Miyake O, Okamoto M, Mikami B, Murata K (2000) Overexpression in Escherichia coli, purification, and characterization of Sphingomonas sp. A1 alginate lyase. Protein Expr Purif 19:84–90CrossRefGoogle Scholar
  20. 20.
    Jana S, Deb JK (2005) Strategies for efficient production of heterologous proteins in Escherichia coli. Appl Microbiol Biotechnol 67:289–298CrossRefGoogle Scholar
  21. 21.
    Schlieker C, Bukau B, Mogk A (2002) Prevention and reversion of protein aggregation by molecular chaperones in the E. coli cytosol: implications for their applicability in biotechnology. J Biotechnol 96:13–21CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Jung Won Shin
    • 1
  • Sung Hee Choi
    • 2
  • Dong Eun Kim
    • 2
  • Hee Sook Kim
    • 2
  • Jae-Hwa Lee
    • 3
  • In Su Lee
    • 4
  • Eun Yeol Lee
    • 1
  1. 1.Department of Chemical Engineering, Industrial Liaison Research CenterKyung Hee UniversityGyeonggi-doRepublic of Korea
  2. 2.Department of Food Science and BiotechnologyKyungsung UniversityPusanRepublic of Korea
  3. 3.Department of Bioscience and BiotechnologySilla UniversityPusanRepublic of Korea
  4. 4.Department of Applied ChemistryKyung Hee UniversityGyeonggi-doRepublic of Korea

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