Bioprocess and Biosystems Engineering

, Volume 33, Issue 9, pp 1059–1065 | Cite as

Biosynthesis of glycerol carbonate from glycerol by lipase in dimethyl carbonate as the solvent

Original Paper


Glycerol carbonate was synthesized from renewable glycerol and dimethyl carbonate using lipase in solvent-free reaction system in which excess dimethyl carbonate played as the reaction medium. A variety of lipases have been tested for their abilities to catalyze transesterification reaction, and Candida antartica lipase B and Novozyme 435 exhibited higher catalytic activities. The silica-coated glycerol with a 1:1 ratio was supplied to prevent two-phase formation between hydrophobic dimethyl carbonate and hydrophilic glycerol. Glycerol carbonate was successfully synthesized with more than 90% conversion from dimethyl carbonate and glycerol with a molar ratio of 10 using Novozyme 435-catalyzed transesterification at 70 °C. The Novozyme 435 [5% (w/w) and 20% (w/w)] and silica gel were more than four times recycled with good stability in a repeated batch operation for the solvent-free synthesis of glycerol carbonate.


Dimethyl carbonate Glycerol Glycerol carbonate Lipase Transesterification 


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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  1. 1.Department of Chemical Engineering, Industrial Liaison Research InstituteKyung Hee UniversityYonginRepublic of Korea

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