Bioprocess and Biosystems Engineering

, Volume 33, Issue 8, pp 961–970 | Cite as

A BOD monitoring disposable reactor with alginate-entrapped bacteria

  • Patricio Villalobos
  • Cristian A. Acevedo
  • Fernando Albornoz
  • Elizabeth Sánchez
  • Erika Valdés
  • Raúl Galindo
  • Manuel E. Young
Original Paper
First Online:
Received:
Accepted:

Abstract

Biochemical oxygen demand (BOD) is a measure of the amount of dissolved oxygen that is required for the biochemical oxidation of the organic compounds in 5 days. New biosensor-based methods have been conducted for a faster determination of BOD. In this study, a mathematical model to evaluate the feasibility of using a BOD sensor, based on disposable alginate-entrapped bacteria, for monitoring BOD in situ was applied. The model considers the influences of alginate bead size and bacterial concentration. The disposable biosensor can be adapted according to specific requirements depending on the organic load contained in the wastewater. Using Klein and Washausen parameter in a Lineweaver–Burk plot, the glucose diffusivity was calculated in 6.4 × 10−10 (m2/s) for beads of 1 mm in diameter and slight diffusion restrictions were observed (n = 0.85). Experimental results showed a correlation (p < 0.05) between the respirometric peak and the standard BOD test. The biosensor response was representative of BOD.

Keywords

BOD Biosensor Alginate bead 

List of symbols

Units in the international system

C

Concentration (kg/m3)

D

Diffusivity (m2/s)

F

Flux of solution in biosensor (m3/s)

Km

Michaelis Menten kinetic constant (kg/m3)

KLa

Volumetric oxygen transfer rate (s−1)

M

Mass of beads (kg)

N

Hill coefficient, dimensionless

P

Volumetric fraction of polymer (m3/m3)

r

Substrate uptake (kg/s kg)

\( \bar{r} \)

Observed substrate uptake (kg/s kg)

R

Radius (m)

t

Time (s)

V

Volume (m3)

X

Biomass concentration (kg/m3)

Y

Yield (kg/kg)

η

Effectiveness factor, dimensionless

Φ

Thiele modulus, dimensionless

Subindex

H

Hill kinetic

H2O

Water

L

Liquid

MAX

Maximum

O2

Oxygen

S

Substrate

SAT

Saturated

X

Biomass

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Notes

Acknowledgments

The authors wish to thank CONICYT (FONDEF Grants D00I1091 and D04T2033) and CORFO (FDI-INNOVA Grant 05CR11 PXT-23.).

References

  1. 1.
    Jia JB, Dong SJ (2003) The progress of microbial biosensors for biochemical oxygen demand. Chin J Anal Chem 31:742–748Google Scholar
  2. 2.
    D’Souza SF (2001) Microbial biosensors. Biosens Bioelectron 16:337–353CrossRefGoogle Scholar
  3. 3.
    Rastogi S, Kumar A, Mehra NK, Makhijani SD, Manoharan A, Gangal V, Kumar R (2003) Development and characterization of a novel immobilized microbial membrane for rapid determination of biochemical oxygen demand load in industrial waste-waters. Biosens Bioelectron 18:23–92CrossRefGoogle Scholar
  4. 4.
    D Souza SF (2001) Microbial biosensors. Biosens Bioelectron 16:337–353CrossRefGoogle Scholar
  5. 5.
    Lin L, Xiao LL, Huang S, Zhao L, Cui JS, Wang XH, Chen X (2006) Novel BOD optical fiber biosensor based on co-immobilized microorganisms in ormosils matrix. Biosens Bioelectron 21:1703–1709CrossRefGoogle Scholar
  6. 6.
    Riedel K, Lehmann M, Tag K, Renneberg R, Kunze G (1998) Arxula adeninivorans based sensor for the estimation of BOD. Anal Lett 31:1–12Google Scholar
  7. 7.
    Vaiopoulou E, Melidis P, Kampragou E, Aivasidis A (2005) On-line load monitoring of wastewaters with a respirographic microbial sensor. Biosens Bioelectron 21:365–371CrossRefGoogle Scholar
  8. 8.
    Karube I, Matsunaga T, Suzuki S (1996) A new disposable sensor for biochemical oxygen demand. Appl Microbiol Biotechnol 46:10–14CrossRefGoogle Scholar
  9. 9.
    Karube I, Mitsuda S, Matsunaga T, Suzuki S (1997) A rapid method for estimation of BOD by using immobilized microbial cells. J Ferment Technol 55:243–248Google Scholar
  10. 10.
    Yoshida N, Yano K, Morita T, McNiven SJ, Nakamura H, Karube I (2000) A mediator-type biosensor as a new approach to biochemical oxygen demand estimation. Analyst 125:2280–2284CrossRefGoogle Scholar
  11. 11.
    Pang HL, Kwok NY, Chan PH, Yeung CH, Lo W, Wong KY (2007) High-throughput determination of biochemical oxygen demand (BOD) by a microplate-based biosensor. Environ Sci Technol 1:4038–4044CrossRefGoogle Scholar
  12. 12.
    Tan TC, Li F, Neoh KG, Lee YK (1992) Microbial membrane modified DO probe for rapid biochemical oxygen demand measurement. Sens Actuators B 8:167–172CrossRefGoogle Scholar
  13. 13.
    Riedel K, Lange K-P, Stein H-J, Kuhn M, Ott P, Scheller F (1990) A microbial sensor for BOD. Water Res 24:883–887CrossRefGoogle Scholar
  14. 14.
    Chan C, Lehmann M, Tag K, Lung M, Kunze G, Riedel K, Gruendig B, Renneberg R (1999) Measurement of biodegradable substances using the salt-tolerant yeast Arxula adeninivorans for a microbial sensor immobilized with poly(carbamoyl) sulfonate (PCS). Part I: construction and characterization of the microbial sensor. Biosens Bioelectron 14:131–138CrossRefGoogle Scholar
  15. 15.
    Lehmann M, Chan C-Y, Lo A, Lung M, Tag K, Kunze G, Riedel K, Gruendig B, Renneberg R (1999) Measurement of biodegradable substances using the salt-tolerant yeast Arxula adeninivorans for a microbial sensor immobilized with poly(carbamoyl) sulfonate (PCS). Part II: application of the novel biosensor to real samples from coastal and island regions. Biosens Bioelectron 14:295–302CrossRefGoogle Scholar
  16. 16.
    Orive G, Hernández R, Gascón A, Igarta M, Pedraz J (2002) Encapsulated cell technology: from research to market. Trends Biotechnol 20:382–387CrossRefGoogle Scholar
  17. 17.
    Huebner H, Buchholz R (1999) Microencapsulation. In: Flickinger MC, Drew SW (eds) Encyclopedia of bioprocess technology: fermentation, biocatalysis and bioseparation. Wiley, New YorkGoogle Scholar
  18. 18.
    Sheu TY, Marshall RT (1993) Microentrapment of lactobacilli in calcium alginate gels. J Food Sci 54:557–561CrossRefGoogle Scholar
  19. 19.
    Tan TC, Li F, Neoh KG (1993) Measurement of BOD by initial rate of response of a microbial sensor. Sens Actuators B 10:137–142CrossRefGoogle Scholar
  20. 20.
    Tan TC, Qian ZR (1997) Dead Bacillus subtilis cells for sensing biochemical oxygen demand of waters and wastewaters. Sens Acutators B 40:65–70CrossRefGoogle Scholar
  21. 21.
    Kumlanghan A, Kanatharana P, Asawatreratanakul P, Mattiasson B, Thavarungkul P (2008) Microbial BOD sensor for monitoring treatment of wastewater from a rubber latex industry. Enz Microbial Technol 42:483–491CrossRefGoogle Scholar
  22. 22.
    Kierstan M, Bucke C (1977) The immobilization of microbial cells subcellular organelles and enzymes in calcium alginate gels. Biotechnol Bioeng 19:387–397CrossRefGoogle Scholar
  23. 23.
    Bettmann H, Rehm HJ (1984) Degradation of phenol by polymer entrapped microorganism. Appl Microbiol Biotechnol 20:285–290CrossRefGoogle Scholar
  24. 24.
    Kim S, Yu S, Son J, Hubner H, Buchholz R (1998) Calculations on O2 transfer in capsules with animal cell for the determination of maximum capsule size without O2 limitation. Biotechnol Lett 20:549–552CrossRefGoogle Scholar
  25. 25.
    Lee K, Heo T (2000) Survival of Bifidobacterium longum immobilized in calcium alginate beads in simulated gastric juices and bile salt solution. Appl Environ Microbiol 66:869–873CrossRefGoogle Scholar
  26. 26.
    David B, Dore E, Jaffrin MY, Legallai C (2004) Mass transfer in a fluidized bed bioreactor using alginate bead for a future bioartificial liver. Int J Artif Organs 27:284–293Google Scholar
  27. 27.
    Gosmann B, Rehm HJ (1986) Oxygen uptake of microorganism entrapped in Ca-alginate. Appl Microbiol Biotechnol 23:163–167CrossRefGoogle Scholar
  28. 28.
    Acevedo CA, Weinstein-Oppenheimer C, Brown DI, Huebner H, Buchholz R, Young ME (2008) A mathematical model for the design of fibrin microcapsules with skin cells. Bioprocess Biosyst Eng doi: 10.1007/s00449-008-0253-1
  29. 29.
    Monier JM, Lindow SE (2003) Differential survival of solitary and aggregated bacterial cells promotes aggregate formation on leaf surfaces. Proc Natl Acad Sci 100:15977–15982CrossRefGoogle Scholar
  30. 30.
    Poulin NM, Mamews JB, Skov KA, Palcic B (1994) Effects of fixation method on image cytometric measurement of DNA content and distribution in cells stained for fluorescence with propidium iodide. J Histochem Cytochem 42:1149–1156Google Scholar
  31. 31.
    Oerther S, Le Gall H, Payan E, Lapicque F, Presle N, Hubert P, Dexheimer J, Netter P, Lapicque F (1999) Hyaluronate-alginate gel as a novel biomaterial: mechanical properties and formation mechanism. Biotechnol Bioeng 63:206–215CrossRefGoogle Scholar
  32. 32.
    APHA (1997) Standard methods for the examination of waters and wastewater, 19th edn. American Public Health Association, Washington, DCGoogle Scholar
  33. 33.
    Markowitz SM, Smith SM, Williams DS (1983) Retrospective analysis of plasmid patterns in a study of burn unit outbreaks of infection due to Enterobacter cloacae. J Infect Dis 148:18–23Google Scholar
  34. 34.
    Flynn DM, Weinstein RA, Nathan C, Gaston MA, Kabins SA (1987) Patients endogenous flora as a source of nosocomial Enterobacter in cardiac surgery. J Infect Dis 156:363–368Google Scholar
  35. 35.
    Sanders CC, Sanders WE (1985) Microbial resistance to newer generation P-lactam antibiotics: clinical and laboratory implications. J Infect Dis 151:399–406Google Scholar
  36. 36.
    Hernández A, Mellado RP, Martínez (1998) Metal Accumulation and Vanadium-Induced Multidrug Resistance by Environmental Isolates of Escherichia hermannii and Enterobacter cloacae. Appl Microbiol Biotechnol 64:4317–4320Google Scholar
  37. 37.
    Yee N, Ma J, Dalia A, Boonfueng T, Kobayashi DI (2007) Se(VI) Reduction and the precipitation of Se(0) by the facultative bacterium Enterobacter cloacae SLD1a-1 are regulated by FNR. Appl Environ Microbiol 73:1914–1920CrossRefGoogle Scholar
  38. 38.
    Krahe M (2002) Biochemical engineering in Ullmann’s Encyclopedia of industrial chemistry, 6th ednGoogle Scholar
  39. 39.
    Wright J, Yang H, Dooley K (1998) Tilapia-A source of hipoxia-resistant islet for encapsulation. Cell Transplant 9:299–307CrossRefGoogle Scholar
  40. 40.
    Robertson GL (1992) Permeability of thermoplastic polymers. In: Robertson GL (ed) Food packaging. Marcel Dekker, New York, pp 73–110Google Scholar
  41. 41.
    Klein J, Washusen P (1979) Diffusion. In: Buchholz K (ed) Characterization of immobilized biocatalysts. Dechema MonographsGoogle Scholar
  42. 42.
    Perry RH, Green DW (1997) Perry’s Chemical Engineers Handbook, 7th edn. McGraw-Hill, New YorkGoogle Scholar
  43. 43.
    Tanaka H, Matsumura M, Veliky IA (1984) Diffusion characteristic of substrates in Ca-alginate gel beads. Biotechnol Bioeng 26:53–58CrossRefGoogle Scholar
  44. 44.
    Hannoum BJM, Stephanopoulos G (1986) Diffusion coefficients of glucose and ethanolin cell-free and cell-occupied calcium alginate membranes. Biotechnol Bioeng 28:829–835CrossRefGoogle Scholar
  45. 45.
    Bailey J, Ollis D (1986) Biochemical engineering fundamentals. McGraw-Hill, New YorkGoogle Scholar
  46. 46.
    Jia J, Tang M, Chen X, Qi L, Dong S (2003) Co-immobilized microbial biosensor for BOD estimation based on sol/gel derived composite material. Biosens Bioelectron 8:1023–1029CrossRefGoogle Scholar
  47. 47.
    Chen JS, Zhang LS, Wang JL (2007) A novel biosensor for the rapid determination of biochemical oxygen demand. Biomed Environ Sci 1:78–83Google Scholar
  48. 48.
    Liu J, Mattiasson B (2002) Microbial sensors for wastewater analysis. Water Res 36:3786–3802CrossRefGoogle Scholar
  49. 49.
    Melidis P, Vaiopoulou E, Aivasidis A (2008) Development and implementation of microbial sensors for efficient process control in wastewater treatment plants. Bioprocess and Biosyst Eng January 10 1615-7605. Published onlineGoogle Scholar
  50. 50.
    Chevakidagarn P (2007) BOD5 Estimation by using UV absorption and COD for rapid industrial effluent monitoring. Environ Monit Assess 131:445–450CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Patricio Villalobos
    • 1
  • Cristian A. Acevedo
    • 1
  • Fernando Albornoz
    • 1
  • Elizabeth Sánchez
    • 1
  • Erika Valdés
    • 2
  • Raúl Galindo
    • 3
  • Manuel E. Young
    • 1
  1. 1.Centro de BiotecnologíaUniversidad Técnica Federico Santa MaríaValparaísoChile
  2. 2.Departamento de QuímicaUniversidad Técnica Federico Santa MaríaValparaísoChile
  3. 3.Departamento de Obras CivilesUniversidad Técnica Federico Santa MaríaValparaísoChile

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