Bioprocess and Biosystems Engineering

, Volume 32, Issue 6, pp 819–824 | Cite as

Xylanases from Aspergillus niger, Aspergillus niveus and Aspergillus ochraceus produced under solid-state fermentation and their application in cellulose pulp bleaching

  • J. H. A. Betini
  • M. Michelin
  • S. C. Peixoto-Nogueira
  • J. A. Jorge
  • H. F. Terenzi
  • M. L. T. M. Polizeli
Original Paper


This study describes the production of xylanases from Aspergillus niveus, A. niger, and A. ochraceus under solid-state fermentation using agro-industrial residues as substrates. Enzyme production was improved using a mixture of wheat bran and yeast extract or peptone. When a mixture of corncob and wheat bran was used, xylanase production from A. niger and A. ochraceus increased by 18%. All cultures were incubated at 30 °C at 70–80% relative humidity for 96 h. For biobleaching assays, 10 or 35 U of xylanase/g dry cellulose pulp were incubated at pH 5.5 for 1 or 2 h, at 55 °C. The delignification efficiency was 20%, the brightness (percentage of ISO) increased two to three points and the viscosity was maintained confirming the absence of cellulolytic activity. These results indicated that the use of xylanases could help to reduce the amount of chlorine compounds used in cellulose pulp treatment.


Aspergillus Cellulase-free xylanases Biobleaching Kappa number Cellulose pulp 



This work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). J.A.J., H·F.T. and M.L.T.M.P. are Research Fellows of CNPq. This work was part of Master Dissertation of J.H.A.B. submitted to the Departamento de Biologia—FFCLRP/USP. The authors thank Ricardo F. Alarcon and Maurício de Oliveira for technical assistance.


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • J. H. A. Betini
    • 1
  • M. Michelin
    • 1
  • S. C. Peixoto-Nogueira
    • 2
  • J. A. Jorge
    • 1
  • H. F. Terenzi
    • 1
  • M. L. T. M. Polizeli
    • 1
  1. 1.Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão PretoUniversidade de São PauloRibeirão Preto, São PauloBrazil
  2. 2.Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão PretoUniversidade de São PauloSão PauloBrazil

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