Bioprocess and Biosystems Engineering

, Volume 32, Issue 6, pp 729–735 | Cite as

Improved glutathione production by gene expression in Pichia pastoris

Original Paper


To utilize Pichia pastoris to produce glutathione, an intracellular expression vector harboring two genes (gsh1 and gsh2) from Saccharomyces cerevisiae encoding enzymes involved in glutathione synthesis and regulated by the glyceraldehyde-3-phosphate dehydrogenase (GAP) promoter was transformed into P. pastoris GS115. Through Zeocin resistance and expression screening, a transformant that had higher glutathione yield (217 mg/L) in flask culture than the host strain was obtained. In fed-batch culture process, this recombinant strain displayed high activity for converting precursor amino acids into glutathione. The glutathione yield and biomass achieved 4.15 g/L and 98.15 g (dry cell weight, DCW)/L, respectively, after 50 h fermentation combined with addition of three amino acids (15 mmol/L glutamic acid, 15 mmol/L cysteine, and 15 mmol/L glycine).


Glutathione production Recombinant Pichia pastoris Fed-batch culture 



This work was supported by Creative Foundation Grant of Shanghai Institute of Pharmaceutical Industry.


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  1. 1.Department of BiochemistryShanghai Institute of Pharmaceutical IndustryShanghaiPeople’s Republic of China

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