Bioprocess and Biosystems Engineering

, Volume 31, Issue 3, pp 241–259

Confocal laser scanning microscopy as an analytical tool in chromatographic research

Mini Review

DOI: 10.1007/s00449-008-0197-5

Cite this article as:
Hubbuch, J. & Kula, M.R. Bioprocess Biosyst Eng (2008) 31: 241. doi:10.1007/s00449-008-0197-5
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Abstract

In recent years, confocal laser scanning microscopy has been developed into a non-invasive tool to probe intra-particle profiles of protein in chromatographic adsorbents. A necessary prerequisite when using this technique lies in the labeling of proteins with fluorescent probes. The quality of the obtained results is thus strongly dependent on the probes used, its sensitivity on experimental parameters and the change of protein characteristics upon binding. In this review, the fundamental issues when using fluorescent probes are described, before giving a critical evaluation on published literature in the field of confocal laser scanning microscopy for the analysis of chromatographic principles.

Keywords

Confocal laser scanning microscopy Chromatography Fluorescent techniques Protein profiling 

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.Institut für Biotechnologie 2Forschungszentrum JülichJülichGermany
  2. 2.Institut für EnzymtechnologieHeinrich-Heine Universität DüsseldorfJülichGermany

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