Metabolic flux and robustness analysis of glycerol metabolism in Klebsiella pneumoniae
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The knowledge of the mechanism of flux distribution will benefit understanding cell physiology and regulation of metabolism. In this study, the measured fluxes obtained under steady-state conditions were used to estimate intracellular fluxes and identify the robustness of branch points of the anaerobic glycerol metabolism in Klebsiella pneumoniae for the production of 1,3-propanediol by metabolic flux analysis. The biomass concentration increased as NADH2/NAD+ decreased at low initial concentration and inversed at high initial glycerol concentration. The flux distribution revealed that the branch points of glycerol and dihydroxyacetonephosphate were rigid to the environmental conditions. However, the pyruvate and acetyl coenzyme A metabolisms gave cells the flexibility to regulate the energy and intermediate fluxes under various environmental conditions. Additionly, it was found that the formation rate of ethanol and the ratio of pyruvate dehydrogenase to pyruvate formate lyase appeared visible fluctuations at high glycerol uptake rate.
Keywords1,3-Propanediol Klebsiella pneumoniae Metabolic flux analysis Glycerol metabolism Metabolic network
This work was supported by the National Natural Science Foundation of China (No. 20576018) and the grant from the Major State Basic Research Development Program of China (973 Program) (No.2007CB714306).
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