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Bioprocess and Biosystems Engineering

, Volume 26, Issue 5, pp 315–323 | Cite as

Agitation effects on submerged growth and product formation of Aspergillus niger

  • Sven Kelly
  • Luis H. Grimm
  • Jan Hengstler
  • Ellen Schultheis
  • Rainer Krull
  • Dietmar C. Hempel
Original Paper

Abstract

Product formation of mycelial organisms, like Aspergillus niger, is intimately connected with their morphology. Pellet morphology is often requested for product formation. Therefore, it is important to reveal the influence of the hydrodynamic conditions on the morphological development. In the present study, pellet morphology and glucoamylase formation were studied under different agitation intensities of A. niger AB1.13. For pellet formation inside the bioreactor, without the use of precultures, it is necessary to work at low energy dissipation rates. Biomass growth and glucoamylase activity were correlated with energy dissipation. Furthermore, product yield was analysed in dependence of pellet size and concentration. The present work shows that simple equations based on Monod-kinetics can describe growth and product formation, in general, also in mycelian organisms. All measured morphological data, like pellet concentration, as well as glucoamylase formation, strongly depend on the hydrodynamic conditions.

Keywords

Aspergillus niger Morphology Glucoamylase production Shear stress Pellet formation 

Abbreviations

α

Growth-associated product formation coefficient (μKat g−1)

β

Non-growth-associated product formation coefficient (μKat g−1h−1)

ε

Energy dissipation (W kg−1)

μ

Specific growth rate (h−1)

μmax

Maximum specific growth rate (h−1)

ρP

Density of wet pellet (kg m−3)

EAGAM

Glucoamylase enzyme activity in the bulk phase (μKat l−1)

CGlu

Glucose concentration (g l−1)

\( C_{{{\text{O}}_{2} }} \)

Oxygen concentration (bulk) (g l−1)

\( C^{ * }_{{{\text{O}}_{2} }} \)

Saturation concentration of oxygen (bulk) (g l−1)

CP

Pellet concentration (l−1)

dP

Pellet diameter (mm)

KGlu

Saturation coefficient glucose (g l−1)

\( K_{{{\text{O}}_{2} }} \)

Saturation coefficient oxygen (g l−1)

kLa

Volumetric gas liquid mass transfer coefficient (h−1)

RGAM

Glucoamylase production rate (μKat l−1h−1)

RGlu

Glucose consumption rate (g l−1h−1)

\( R_{{{\text{O}}_{2} }} \)

Oxygen consumption rate (g l−1h−1)

RX

Biomass growth rate (g l−1h−1)

X

Biomass concentration (g l−1)

YOX

Yield coefficient of biomass on oxygen (g g−1)

YSX

Yield coefficient of biomass on glucose (g g−1)

Notes

Acknowledgements

The authors acknowledge financial support provided by the German Research Foundation. This project is part of the SFB 578 “Development of biotechnological processes by integrating genetic and engineering methods—from gene to product”.

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Sven Kelly
    • 1
  • Luis H. Grimm
    • 1
  • Jan Hengstler
    • 1
  • Ellen Schultheis
    • 1
  • Rainer Krull
    • 1
  • Dietmar C. Hempel
    • 1
  1. 1.Institute of Biochemical EngineeringTechnical University of BraunschweigBraunschweigGermany

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