Bioprocess and Biosystems Engineering

, Volume 26, Issue 3, pp 147–150 | Cite as

High cell density fed-batch cultivation of Escherichia coli using exponential feeding combined with pH-stat

  • Beom Soo Kim
  • Seung Chul Lee
  • Sang Yup Lee
  • Yong Keun Chang
  • Ho Nam Chang
Original Paper


A new feeding strategy in fed-batch culture, exponential feeding combined with pH-stat is suggested to avoid the accumulation of substrate in culture broth. Exponential feeding was stopped whenever a predetermined amount of limiting substrate was supplied and then pH change was observed. When pH rose above an upper limit due to the depletion of substrate, feeding was restarted. With this feeding strategy, recombinant Escherichia coli could be grown to 101 g/l by controlling the specific growth rate at 0.1 h−1.


Fed-batch Exponential feeding pH-stat Escherichia coli 


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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Beom Soo Kim
    • 1
  • Seung Chul Lee
    • 1
  • Sang Yup Lee
    • 1
  • Yong Keun Chang
    • 1
  • Ho Nam Chang
    • 1
  1. 1.Department of Chemical EngineeringChungbuk National UniversityCheongjuKorea

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