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Oecologia

, Volume 187, Issue 1, pp 1–13 | Cite as

Assessing seasonal changes in animal diets with stable-isotope analysis of amino acids: a migratory boreal songbird switches diet over its annual cycle

  • Camila Gómez
  • Thomas Larsen
  • Brian Popp
  • Keith A. Hobson
  • Carlos Daniel Cadena
Highlighted Student Research

Abstract

Tools to study seasonal changes in animal diets are needed to address a wide range of ecological questions. This is especially true of migratory animals that experience distinct environments where diets may be substantially different. However, tracking diets of individuals that move vast distances has proven difficult. Compound-specific isotope analysis has emerged as a valuable tool to study diets but has been little used to study dietary changes of migratory animals. Using this technique, we quantify seasonal variation in the annual diet of a migratory songbird (gray-cheeked thrush, Catharus minimus) and test the hypothesis that migrants change their diet in response to the energetic requirements of different periods of the annual cycle. By measuring δ13C and δ15N values of amino acids from feathers grown on the breeding grounds, blood formed during migration and claw grown on the wintering grounds, we found that migration is associated with greater consumption of fruit, compared to the breeding or wintering periods. This was confirmed by the lower trophic position of blood compared to feather and claw, by a decrease in the δ15N value of the source amino acid phenylalanine in blood as a function of days of stopover, and by the positive correlation between δ15N and δ13C values of phenylalanine in blood, and not in feather or claw. This study illustrates how isotopic analysis of amino acids can contribute to understand food webs, seasonal dietary changes and metabolic routing of nutrients in migratory animals.

Keywords

Amino acids Compound specific δ13C and δ15Dietary routing Food web Gray-cheeked thrush Migration Trophic position 

Notes

Acknowledgements

We thank Natalie Wallsgrove for her training and help in the lab, and Nick Bayly, Jeyson Sanabria, Laura Céspedes and Paulo Pulgarín for their support in the field. We also thank Nathan Cooper, Seth Newsome and two anonymous reviewers for their comments, which helped us improve previous versions of this manuscript. All applicable institutional and/or national guidelines for the care and use of animals were followed. This is SOEST Contribution number 10332.

Author contribution statement

CG, BP, KAH and CDC designed the study and CG collected the samples. CG, TL and KH ran laboratory analyses. CG analyzed the data, led the writing of the manuscript and all authors contributed critically to the drafts and gave approval for publication.

Funding

This study was funded by a ‘research in residence’ award from the University of Utah (NSF award #1137336 for inter-university training in continental-scale ecology), by Colciencias (‘Ciencias Básicas’ Grant no. 658), by the Universidad de Los Andes (Proyecto Semilla) and by a Memorial fund Grant from the Eastern Bird Banding Association. TL was supported by the DFG funded Cluster of Excellence 80 “The Future Ocean”.

Supplementary material

442_2018_4113_MOESM1_ESM.docx (1.3 mb)
Supplementary material 1 (DOCX 1339 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Laboratorio de Biología Evolutiva de Vertebrados, Departamento de Ciencias BiológicasUniversidad de Los AndesBogotáColombia
  2. 2.SELVA: Investigación para conservación en el NeotrópicoBogotáColombia
  3. 3.Leibniz-Laboratory for Radiometric Dating and Stable Isotope ResearchChristian-Albrechts-Universität zu KielKielGermany
  4. 4.Department of Geology and GeophysicsUniversity of HawaiiManoaUSA
  5. 5.Department of BiologyUniversity of Western OntarioLondonCanada

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