Effects of atmospheric CO2 concentration, irradiance, and soil nitrogen availability on leaf photosynthetic traits of Polygonum sachalinense around natural CO2 springs in northern Japan
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Long-term exposure to elevated CO2 concentration will affect the traits of wild plants in association with other environmental factors. We investigated multiple effects of atmospheric CO2 concentration, irradiance, and soil N availability on the leaf photosynthetic traits of a herbaceous species, Polygonum sachalinense, growing around natural CO2 springs in northern Japan. Atmospheric CO2 concentration and its interaction with irradiance and soil N availability affected several leaf traits. Leaf mass per unit area increased and N per mass decreased with increasing CO2 and irradiance. Leaf N per area increased with increasing soil N availability at higher CO2 concentrations. The photosynthetic rate under growth CO2 conditions increased with increasing irradiance and CO2, and with increasing soil N at higher CO2 concentrations. The maximal velocity of ribulose 1,5-bisphosphate carboxylation (Vcmax) was affected by the interaction of CO2 and soil N, suggesting that down-regulation of photosynthesis at elevated CO2 was more evident at lower soil N availability. The ratio of the maximum rate of electron transport to Vcmax (Jmax/Vcmax) increased with increasing CO2, suggesting that the plants used N efficiently for photosynthesis at high CO2 concentrations by changes in N partitioning. To what extent elevated CO2 influenced plant traits depended on other environmental factors. As wild plants are subject to a wide range of light and nutrient availability, our results highlight the importance of these environmental factors when the effects of elevated CO2 on plants are evaluated.
KeywordsLeaf photosynthesis Light availability Natural CO2 springs Nitrogen partitioning Soil nitrogen availability
We thank the landowner (Hakkoda-Onsen, Tashiro Bokuya-Chikusan Kumiai) for permission to use the site for this study, Aki Shigeno for her help in field measurements, Riichi Oguchi for his help in anatomical analysis, and Onno Muller, Satoki Sakai, and Naoko Tokuchi for their valuable comments. This study was supported in part by grants from the Japan Ministry of Education, Culture, Sports, Science and Technology (18770011 and 21780140), and the Global Environment Research Fund (F-052) from the Japan Ministry of the Environment, and by the Sumitomo Foundation (073130).
- Ainsworth EA, Davey PA, Hymus GJ, Osborne CP, Rogers A, Blum H, Nösberger J, Long SP (2003) Is stimulation of leaf photosynthesis by elevated carbon dioxide concentration maintained in the long term? A test with Lolium perenne grown for 10 years at two nitrogen fertilization levels under free air CO2 enrichment (FACE). Plant Cell Environ 26:705–714CrossRefGoogle Scholar
- Badiani M, Raschi A, Paolacci AR, Miglietta F (2000) Plant responses to elevated CO2; a perspective from natural CO2 springs. In: Agrawal SB, Agrawal M (eds) Environmental pollution and plant responses. Lewis, Florida, pp 45–81Google Scholar
- Fernandez MD, Pieters A, Donoso C, Tezara W, Azkue M, Herrera C, Rengifo E, Herrera A (1998) Effects of a natural source of very high CO2 concentration on the leaf gas exchange, xylem water potential and stomatal characteristics of plants of Spatiphylum cannifolium and Bauhinia multinervia. New Phytol 138:689–697CrossRefGoogle Scholar
- Keeney DR, Nelson DW (1982) Nitrogen-inorganic forms. In: Page AL (ed) Methods of soil analysis, part 2. American Society of Agronomy, Madison, pp 643–698Google Scholar
- Koch GW (1994) The use of natural situations of CO2 enrichment in studies of vegetation responses to increasing atmospheric CO2. In: Schulze ED, Mooney HA (eds) Design and execution of experiments on CO2 enrichment. Commission of the European Communities, Luxembourg, pp 318–392Google Scholar
- Medlyn BE, Badeck FW, de Pury DGG, Barton CVM, Broadmeadow M, Ceulemans R, de Angelis P, Forstreuter M, Jach ME, Kellomaki S, Laitat E, Marek M, Philippot S, Rey A, Strassemeyer J, Laitinen K, Liozon R, Portier B, Roberntz P, Wang K, Jarvis PG (1999) Effects of elevated CO2 on photosynthesis in European forest species: a meta-analysis of model parameters. Plant Cell Environ 22:1475–1495CrossRefGoogle Scholar
- Miglietta F, Raschi A, Bettarini I, Badiani M, van Gardingen P (1994) Carbon dioxide springs and their use for experimentation. In: Schulze ED, Mooney HA (eds) Design and execution of experiments on CO2 enrichment. Commission of the European Communities, Luxembourg, pp 393–403Google Scholar
- Porra RJ, Thompson WA, Kriedemann PE (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophyll a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochim Biophys Acta 975:384–394CrossRefGoogle Scholar
- Sokal RR, Rohlf FJ (1995) Biometry. Freeman, New YorkGoogle Scholar