Rearing environment effects on immune defence in blue tit Cyanistes caeruleus nestlings
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Rearing conditions may influence ontogeny and functioning of the immune system. Activation of different mechanisms involved in host disease resistance and their internal regulation can be affected by intrinsic and extrinsic factors influencing development. I investigated how rearing environment can influence associations between humoral and cellular constituents of immune defence in nestling blue tits (Cyanistes caeruleus). The ability to mount a cell-mediated immune response was estimated as a hypersensitivity reaction to phytohaemagglutinin, and the ontogeny of humoral immunity was determined by assessing circulating levels of total IgG in 15-day-old nestlings. Heterogeneity in rearing conditions was evoked by placing nest-boxes in areas differing in habitat structural characteristics, and examining natural variation in nest ectoparasite infestations, hatching date, brood size and brood sex-ratio. Habitat characteristics, parasitism and hatching date may shape associations between different components of the immune system in developing birds. I discuss the effects of rearing conditions on the interaction between different arms of the immune system and the implications for understanding negative correlations within the immune system at the individual and brood level.
KeywordsCirculating immunoglobulins Ectoparasites Forest structure Immune function Phytohaemagglutinin
I thank J. Moreno, A.P. Møller and two anonymous reviewers for valuable comments on the manuscript. The study was supported financially by projects 07M/0137/2000 (Comunidad de Madrid) to L.M. Carrascal and CGL2004-00787/BOS to J. Moreno (Ministerio de Ciencia y Tecnología). E.A. was supported by a fellowship from El Ventorrillo-CSIC during field and laboratory work. During final analyses and writing, support to E.A. was provided by Laboratoire de Parasitologie Evolutive UPMC UMR 7103, France. J.A. Fargallo, J.J. Sanz, S. Merino and A. Martin were of great help in the field and in different phases of the project. I thank J. Martinez, J.A. Davila and A. Machordon for advice in laboratory techniques. Museo Nacional de Ciencias Naturales (CSIC) and El-Ventorrillo field station provided infrastructure and logistical support during field and laboratory work. Capture of birds and fieldwork in the study area was conducted under authorization from Comunidad de Madrid and Ministerio de Medio Ambiente, Spain.
- Apanius V (1998) The immune system. In: Starck JM, Ricklefs RE (eds) Avian growth and development. Oxford University Press, New York, pp 203–222Google Scholar
- Ardia DR (2007) The ability to mount multiple immune responses simultaneously varies across the range of the tree swallow. Ecography 30:23–30Google Scholar
- Blondel J, Dias PC, Maistre M, Perret P (1993) Habitat heterogeneity and life-history variation in Mediterranean blue tits (Parus caeruleus). Auk 110:511–520Google Scholar
- Brokaw NVL, Lent RA (1999) Vertical structure. In: Hunter ML (ed) Maintaining biodiversity in forest ecosystems. Cambridge University Press, Cambridge, pp 373–399Google Scholar
- Garvin MC, Remsen JV (1997) An alternative hypothesis for heavier parasite loads of brightly colored birds: exposure at the nest. Auk 114:179–191Google Scholar
- Gershwin ME, Beach RS, Hurley LS (1985) Nutrition and immunity. Academic, OrlandoGoogle Scholar
- Roit IM, Brostoff J, Male DK (1998) Immunology. Mosby, LondonGoogle Scholar
- Rosenthal R (1994) Parametric measures of effect size. In: Cooper H, Hedges LV (eds) The handbook of research synthesis. Russell Sage Foundation, New York, pp 231–244Google Scholar
- Wakelin D (1996) Immunity to parasites. Cambridge University Press, CambridgeGoogle Scholar