Oecologia

, Volume 157, Issue 4, pp 629–640 | Cite as

Identifying the predator complex of Homalodisca vitripennis (Hemiptera: Cicadellidae): a comparative study of the efficacy of an ELISA and PCR gut content assay

  • Valerie Fournier
  • James Hagler
  • Kent Daane
  • Jesse de León
  • Russell Groves
Community Ecology - Methods Paper

Abstract

A growing number of ecologists are using molecular gut content assays to qualitatively measure predation. The two most popular gut content assays are immunoassays employing pest-specific monoclonal antibodies (mAb) and polymerase chain reaction (PCR) assays employing pest-specific DNA. Here, we present results from the first study to simultaneously use both methods to identify predators of the glassy winged sharpshooter (GWSS), Homalodisca vitripennis (Germar) (Hemiptera: Cicadellidae). A total of 1,229 arthropod predators, representing 30 taxa, were collected from urban landscapes in central California and assayed first by means of enzyme-linked immunosorbent assay (ELISA) using a GWSS egg-specific mAb and then by PCR using a GWSS-specific DNA marker that amplifies a 197-base pair fragment of its cytochrome oxidase gene (subunit I). The gut content analyses revealed that GWSS remains were present in 15.5% of the predators examined, with 18% of the spiders and 11% of the insect predators testing positive. Common spider predators included members of the Salticidae, Clubionidae, Anyphaenidae, Miturgidae, and Corinnidae families. Common insect predators included lacewings (Neuroptera: Chrysopidae), praying mantis (Mantodea: Mantidae), ants (Hymenoptera: Formicidae), assassin bugs (Hemiptera: Reduviidae), and damsel bugs (Hemiptera: Nabidae). Comparison of the two assays indicated that they were not equally effective at detecting GWSS remains in predator guts. The advantages of combining the attributes of both types of assays to more precisely assess field predation and the pros and cons of each assay for mass-screening predators are discussed.

Keywords

Conservation biological control ELISA Generalist predators Gut content PCR Predator-prey interactions Spiders 

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Valerie Fournier
    • 1
    • 2
    • 5
  • James Hagler
    • 2
    • 7
  • Kent Daane
    • 1
  • Jesse de León
    • 3
  • Russell Groves
    • 4
    • 6
  1. 1.Department of Environmental Science, Policy and ManagementUniversity of CaliforniaBerkeleyUSA
  2. 2.Arid-Land Agricultural Research Center, USDA-ARSMaricopaUSA
  3. 3.Beneficial Insects Research Unit, USDA-ARSWeslacoUSA
  4. 4.Exotic and Invasive Diseases and Pests Lab, USDA-ARSParlierUSA
  5. 5.Département de PhytologieUniversité LavalQuebecCanada
  6. 6.Department of EntomologyUniversity of WisconsinMadisonUSA
  7. 7.USDA-ARSMaricopaUSA

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