Oecologia

, Volume 151, Issue 4, pp 687–696

Stoichiometric response of nitrogen-fixing and non-fixing dicots to manipulations of CO2, nitrogen, and diversity

  • Amy M. Novotny
  • John D. Schade
  • Sarah E. Hobbie
  • Adam D. Kay
  • Marcia Kyle
  • Peter B. Reich
  • James J. Elser
Global Change and Conservation Ecology

Abstract

Human activities have resulted in increased nitrogen deposition and atmospheric CO2 concentrations in the biosphere, potentially causing significant changes in many ecological processes. In addition to these ongoing perturbations of the abiotic environment, human-induced losses of biodiversity are also of major concern and may interact in important ways with biogeochemical perturbations to affect ecosystem structure and function. We have evaluated the effects of these perturbations on plant biomass stoichiometric composition (C:N:P ratios) within the framework of the BioCON experimental setup (biodiversity, CO2, N) conducted at the Cedar Creek Natural History Area, Minnesota. Here we present data for five plant species: Solidago rigida, Achillea millefolium, Amorpha canescens, Lespedeza capitata, and Lupinus perennis. We found significantly higher C:N and C:P ratios under elevated CO2 treatments, but species responded idiosyncratically to the treatment. Nitrogen addition decreased C:N ratios, but this response was greater in the ambient CO2 treatments than under elevated CO2. Higher plant species diversity generally lowered both C:N and C:P ratios. Importantly, increased diversity also led to a more modest increase in the C:N ratio with elevated CO2 levels. In addition, legumes exhibited lower C:N and higher C:P and N:P ratios than non-legumes, highlighting the effect of physiological characteristics defining plant functional types. These data suggest that atmospheric CO2 levels, N availability, and plant species diversity interact to affect both aboveground and belowground processes by altering plant elemental composition.

Keywords

BioCON Ecological stoichiometry Elevated CO2 Nitrogen enrichment Species richness 

References

  1. Aerts R, Chapin FS (2000) The mineral nutrition of wild plants revisited: a re-evaluation of processes and patterns. Adv Ecol Res 30:1–67CrossRefGoogle Scholar
  2. Billings SA et al (2003) Effects of elevated carbon dioxide on green leaf tissue and leaf litter quality in an intact Mojave Desert ecosystem. Global Change Biol 9:729–735CrossRefGoogle Scholar
  3. Cannell M, Thornley J (1998) N-poor ecosystems may respond more to elevated [CO2] than N-rich ones in the long term. A model analysis of grassland. Global Change Biol 4:431–442CrossRefGoogle Scholar
  4. Cebrian J (1999) Patterns in the fate of production in plant communities. Am Nat 154:449–468PubMedCrossRefGoogle Scholar
  5. De Angelis P, Chigwerewe KS, Mugnozza GES (2000) Litter quality and decomposition in a CO2-enriched Mediterranean forest ecosystem. Plant Soil 224:31–41CrossRefGoogle Scholar
  6. Dukes JS, Hungate BA (2002) Elevated carbon dioxide and litter decomposition in California annual grasslands: which mechanisms matter? Ecosystems 5:171–183CrossRefGoogle Scholar
  7. Elser JJ, Sterner RW, Gorokhova E, Fagan WF et al. (2000) Biological stoichiometry from genes to ecosystems. Ecol Lett 3:540–550Google Scholar
  8. Elser JJ, Marzolf ER, Goldman CR (1990) Phosphorus and nitrogen limitation of phytoplankton growth in the freshwaters of North America: a review and critique of experimental enrichments. Can J Fish Aquat Sci 47:1468–1477CrossRefGoogle Scholar
  9. Enriquez S et al. (1993) Patterns in decomposition rates among photosynthetic organisms: the importance of detritus C:N:P content. Oecologia 94:457–471CrossRefGoogle Scholar
  10. Gifford RM, Barrett DJ, Lutze JL (2000) The effects of elevated [CO2] on the C:N and C:P mass ratios of plant tissues. Plant Soil 224:1–4CrossRefGoogle Scholar
  11. Hamilton JG, Zangerl AR, Berenbaum MR, Pippen J et al. (2004) Insect herbivory in an intact forest understory under experimental CO2 enrichment. Oecologia 138:566–573Google Scholar
  12. Hobbie SE (1992) Effects of plant species on nutrient cycling. Tree 7:336–339Google Scholar
  13. Kerkhoff A et al. (2006) Phylogenetic and functional variation in the scaling of nitrogen and phosphorus in the seed plants. Am Nat 168:E103–E122PubMedCrossRefGoogle Scholar
  14. Killingbeck KT (1996) Nutrients in senesced leaves: keys to the search for potential resorption and resorption proficiency. Ecology 77:1716–1727CrossRefGoogle Scholar
  15. King JY et al. (2004) Plant nitrogen dynamics in shortgrass steppe under elevated atmospheric carbon dioxide. Ecosystems 7:147–160Google Scholar
  16. Kobe RK, Lepczyk CA, Iyer M (2005) Resorption efficiency decreases with increasing green leaf nutrients in a global data set. Ecology 86:2780–2792Google Scholar
  17. Lee TA et al. (2003) Contrasting growth response of an N2-fixing and non-fixing forb to elevated CO2: dependence on soil N supply. Plant Soil 255:475–486CrossRefGoogle Scholar
  18. Liao JX, Hou ZD, Wang GX (2002) Effects of elevated CO2 and drought on chemical composition and decomposition of spring wheat (Triticum aestivum). Funct Plant Biol 29:891–897CrossRefGoogle Scholar
  19. Lüscher A, Hendrey GR, Nösberger J (1998) Long-term responsiveness to free air CO2 enrichment of functional types, species and genotypes of plants from fertile permanent grassland. Oecologia 113:37–45Google Scholar
  20. Marschner H (1995) Mineral nutrition of higher plants. Academic, LondonGoogle Scholar
  21. Niklaus PA, Spinnlet D, Korner Ch (1998) Nutrient relations in calcareous grassland under elevated CO2. Oecologia 116:67–75Google Scholar
  22. Norby RJ (1998) Nitrogen deposition: a component of global change analyses. New Phytol 139:189–200CrossRefGoogle Scholar
  23. Norby RJ, Cotrufu MF, Ineson P et al. (2001) Elevated CO2, litter chemistry, and decomposition: a synthesis. Oecologia 127:153–165Google Scholar
  24. Perkins MC, Woods HA, Harrison JF, Elser JJ (2004) Dietary phosphorus affects the growth of larval Manduca sexta. Arch Insect Biochem Physiol 55:153–168PubMedCrossRefGoogle Scholar
  25. Reich PB, Knops T, Tilman D et al. (2001a) Do species and functional groups differ in acquisition and use of C, N and water under varying atmospheric CO2 and N deposition regimes? A field test with 16 grassland species. New Phytol 150:435–448Google Scholar
  26. Reich PB, Timan D, Craine J et al. (2001b) Plant diversity enhances ecosystem responses to elevated CO2 and nitrogen deposition. Nature 410:809–812Google Scholar
  27. Reich PB, Tilman D, Naeem S, Ellsworth DS et al. (2004) Species and functional group diversity independently influence biomass accumulation and its response to CO2 and N. Proc Natl Acad Sci USA 101:10101–10106Google Scholar
  28. Rueth HM, Baron JS (2002) Differences in Englemann spruce forest biogeochemistry east and west of the Continental Divide in Colorado, USA. Ecosystems 5:45–57CrossRefGoogle Scholar
  29. Sala OE et al. (2000) Global biodiversity scenarios for the year 2100. Science 287:1770PubMedCrossRefGoogle Scholar
  30. Schade JD et al. (2003) Stoichiometric tracking of soil nutrients by a desert insect herbivore. Ecol Lett 6:96–101CrossRefGoogle Scholar
  31. Schadler M et al. (2003) Palatability, decomposition and insect herbivory: patterns in a successional old-field plant community. Oikos 103:121–132CrossRefGoogle Scholar
  32. Sterner RW, Elser JJ (2002) Ecological stoichiometry: the biology of elements from molecules to the biosphere. Princeton, New JerseyGoogle Scholar
  33. Stiling P et al. (2003) Elevated CO2 lowers relative and absolute herbivore density across all species of a scrub-oak forest. Oecologia 134:82–87PubMedCrossRefGoogle Scholar
  34. Sundareshwar PV, Morris JT, Koepfler EK, Fornwalt B (2003) Phosphorus limitation of coastal ecosystem processes. Science 299:563–565PubMedCrossRefGoogle Scholar
  35. Torbert HA, Prior SA, Rogers HH et al. (2004) Elevated atmospheric CO2 effects on N fertilization in grain sorghum and soybean. Field Crops Res 88:57–67Google Scholar
  36. Vitousek PM (1982) Nutrient cycling and nutrient use efficiency. Am Nat 119:553–572CrossRefGoogle Scholar
  37. Vitousek PM (1994) Beyond global warming: ecology and global change. Ecology 75:1861–1876CrossRefGoogle Scholar
  38. Vitousek PM, Howarth RW (1991) Nitrogen limitation on land and in the sea: how can it occur? Biogeochemistry 13:87–115CrossRefGoogle Scholar
  39. White TCR (1993) The inadequate environment: nitrogen and the abundance of animals. Springer, Berlin Heidelberg New YorkGoogle Scholar
  40. Winker JB, Herbst M (2004) Do plants of a semi-natural grassland community benefit from long-term CO2 enrichment? Basic Appl Ecol 5:131–143CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Amy M. Novotny
    • 1
  • John D. Schade
    • 2
  • Sarah E. Hobbie
    • 3
  • Adam D. Kay
    • 4
  • Marcia Kyle
    • 1
  • Peter B. Reich
    • 3
  • James J. Elser
    • 1
  1. 1.School of Life SciencesArizona State UniversityTempeUSA
  2. 2.Environmental StudiesSt Olaf CollegeNorthfieldUSA
  3. 3.Department of Ecology, Evolution, and BehaviorUniversity of MinnesotaSt PaulUSA
  4. 4.Department of BiologyUniversity of St ThomasSt PaulUSA

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