Advertisement

Cell and Tissue Research

, Volume 298, Issue 2, pp 287–293 | Cite as

Immunocytochemical evidence for a paracrine interaction between GIP and GLP-1-producing cells in canine small intestine

  • Anders B. Damholt
  • Hans Kofod
  • Alison M.J. Buchan
Regular Article

Abstract.

Glucagon-like-peptide 1 (GLP-1) released from the intestine is considered to be an important incretin. We have recently demonstrated that glucose-dependent insulinotropic peptide (GIP) stimulated GLP-1 secretion from canine ileal L cells in culture. To investigate further the interplay between GLP-1- and GIP-secreting cells, we set out to determine the exact location and abundance of both cell types throughout the canine intestine. Canine small intestine was subdivided into 15–20 segments and investigated by immunocytochemistry with computer-assisted imaging. The abundance of GIP-, GLP-1- and somatostatin-immunoreactive cells was determined. GIP-secreting K cells were equally distributed in duodenum and jejunum, with the GLP-1-secreting L cells concentrated in the jejunum (5% duodenum, 73% jejunum and 22% ileum). These results indicated that the middle section of the small intestine containing 69% of the K cells also contained 51% of the L cells. Double immunostaining confirmed this overlap and furthermore over 30% of the L cells in this region were found adjacent to K cells. These results suggest the existence of a paracrine interaction between the K and L cells and indicate the importance of the jejunum in the regulation of insulin release by enteric-derived incretins.

Somatostatin Enteroinsular axis Immunocytochemistry Dog 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag 1999

Authors and Affiliations

  • Anders B. Damholt
    • 1
  • Hans Kofod
    • 1
  • Alison M.J. Buchan
    • 2
  1. 1.Diabetes Discovery, Novo Nordisk A/S, Novo allé 6A1.57, Bagsvaerd DK-2880, Denmark
  2. 2.Department of Physiology, University of British Columbia, 2146 Health Sciences Mall, Vancouver, BC, Canada, V6T 1Z3

Personalised recommendations