Cell and Tissue Research

, Volume 292, Issue 2, pp 211–218 | Cite as

Calcium-binding proteins in the retina of a calbindin-null mutant mouse

  • H. Wässle
  • L. Peichl
  • Matti S. Airaksinen
  • Michael Meyer
REGULAR ARTICLE

Abstract 

Calcium-binding proteins are abundantly expressed in many neurons of mammalian retinae. Their physiological roles are, however, largely unknown. This is particularly true for calcium-modulating proteins (“calcium buffers”) such as calbindin D28k. Here, we have studied retinae of wildtype (+/+) and calbindin-null mutant (–/–) mice by using immunocytochemical methods. Although calbindin immunoreactivity was completely absent in the calbindin (–/–) retinae, those cells that express the protein in wildtype retinae, such as horizontal cells, were still present and appeared normal. This was verified by immunostaining horizontal cells for various neurofilament proteins. In order to assess whether other calcium-binding proteins are upregulated in the mutant mouse and may thus compensate for the loss of calbindin, mouse retinae were also immunolabeled for parvalbumin, calretinin, and a calmodulin-like protein (CALP). In no instance could a change in the expression pattern of these proteins be detected by immunocytochemical methods. Thus, our results show that calbindin is not required for the maintenance of the light-microscopic structure of the differentiated retina and suggest roles for this protein in retinal function.

Key words Calbindin Parvalbumin Calretinin Neurofilament protein Calmodulin-like protein Mouse [calbindin null mutant ( )] 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • H. Wässle
    • 1
  • L. Peichl
    • 1
  • Matti S. Airaksinen
    • 2
  • Michael Meyer
    • 2
  1. 1.Max-Planck-Institut für Hirnforschung, Neuroanatomie, Deutschordenstrasse 46, D-60528 Frankfurt a.M., Germany Tel.: +49 69 96769–211; Fax: +49 69 96769–206; e-mail: waessle@mpih-frankfurt.mpg.deDE
  2. 2.Max-Planck-Institut für Psychiatrie, Am Klopferspitz 18a, D-82152 Martinsried, GermanyDE

Personalised recommendations