Cell and Tissue Research

, Volume 291, Issue 3, pp 575–579

Polysome-like structures in the chromatoid body of rat spermatids

  • Jaime Figueroa
  • Luis O. Burzio
SHORT COMMUNICATION

Abstract 

A procedure for isolating the chromatoid body from the testis of 40-day-old rats was developed. Electron-microscopical analysis indicated that about 70% of the isolated organelles were chromatoid bodies, while the remaining structures corresponded to dense bodies and probably to satellites. Negative staining of the isolated organelles revealed the presence of polysome-like structures in about 20% of the chromatoid bodies suggesting that the polysomes were not due to contamination with cytoplasmic polysomes. Moreover, the presence of RNA in the stroma of the chromatoid body was confirmed by RNAse-gold staining. Preliminary electrophoretic analysis of the RNA extracted from the organelles revealed the presence of a complex population of RNAs including 5.8 and 5 S ribosomal RNAs but no tRNA.

Key words Chromatoid body Polysomes RNA Spermatid Spermatogenesis Rat 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Jaime Figueroa
    • 1
  • Luis O. Burzio
    • 1
  1. 1.Instituto de Bioquímica, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, ChileCL
  2. 2.BIOS CHILE Ingeniería Genética S.A., Avenida Marathon 1943, Ñuñoa – Santiago, Chile Tel.: 56–2–238 1878; Fax: 56–2–238 4250CL

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