Cell and Tissue Research

, Volume 290, Issue 1, pp 1–10

GAL4-responsive UAS-tau as a tool for studying the anatomy and development of the Drosophila central nervous system

  • Kei Ito
  • Heinz Sass
  • Joachim Urban
  • Alois Hofbauer
  • Stephan Schneuwly
Regular articles

DOI: 10.1007/s004410050901

Cite this article as:
Ito, K., Sass, H., Urban, J. et al. Cell Tissue Res (1997) 290: 1. doi:10.1007/s004410050901

Abstract. 

To improve the quality of cytoplasmic labelling of GAL4-expressing cells in Drosophila enhancer-trap and transgenic strains, a new GAL4-responsive reporter UAS-tau, which features a bovine tau cDNA under control of a yeast upstream activation sequence (UAS), was tested. Tau, a microtubule-associated protein, is distributed actively and evenly into all cellular processes. Monoclonal anti-bovine Tau antibody reveals the axonal structure of the labelled cells with detail similar to that of Golgi impregnation. We demonstrate that the UAS-tau system is especially useful for studying processes of differentiation and reorganisation of identified neurones during postembryonic development.

Key words: Brain Postembryonic development Metamorphosis Neurone Enhancer trap Drosophila melanogaster (Insecta) 

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Kei Ito
    • 1
  • Heinz Sass
    • 1
  • Joachim Urban
    • 1
  • Alois Hofbauer
    • 2
  • Stephan Schneuwly
    • 2
  1. 1.Institut für Genetik, Universität Mainz, Saarstr. 21, D-55122 Mainz, GermanyDE
  2. 2.Lehrstuhl für Biologie VI, Institut für Zoologie, Universität Regensburg, Universitätsstr. 31, D-93040 Regensburg, GermanyDE

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