Cell and Tissue Research

, Volume 283, Issue 1, pp 159–165

Expression of neural cell adhesion molecule (N-CAM) in perisinusoidal stellate cells of the human liver

  • Kazuki Nakatani
  • Shuichi Seki
  • Norifumi Kawada
  • Kenzo Kobayashi
  • Kenji Kaneda

DOI: 10.1007/s004410050524

Cite this article as:
Nakatani, K., Seki, S., Kawada, N. et al. Cell Tissue Res (1995) 283: 159. doi:10.1007/s004410050524
  • 62 Downloads

Abstract.

Neural cell adhesion molecule (N-CAM) is distributed in most nerve cells and some non-neural tissues. The present immunohistochemical study has revealed, for the first time, the expression of N-CAM in perisinusoidal stellate cells of the human liver. Liver specimens were stained with monoclonal antibody against human Leu19 (N-CAM) by a streptoavidin-biotin-peroxidase-complex method. Light- and electron-microscopic analyses have shown that N-CAM-positive nerve fibers are distributed in the periportal and intermediate zones of the liver lobule. Perisinusoidal stellate cells in these zones are also positive for N-CAM. N-CAM is expressed on the surface of the cell, including cytoplasmic projections. Close contact of N-CAM-positive nerve endings with N-CAM-positive stellate cells has been observed. On the other hand, stellate cells in the centrilobular zone exhibit weak or no reaction for N-CAM. Perivascular smooth muscle cells and fibroblasts in the portal area and myofibroblasts around the central veins are negative for N-CAM. The present results indicate that the perisinusoidal stellate cells in the periportal and intermediate zones of the liver lobule characteristically express N-CAM, unlike other related mesenchymal cells, and suggest that the intralobular heterogeneity of N-CAM expression by stellate cells is related to the different maturational stages of these cells.

Key words: Cell adhesion molecules neuronal Stellate cells Liver Immunohistochemistry Human 

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • Kazuki Nakatani
    • 1
  • Shuichi Seki
    • 2
  • Norifumi Kawada
    • 2
  • Kenzo Kobayashi
    • 2
  • Kenji Kaneda
    • 1
  1. 1.Second Department of Anatomy, Osaka City University Medical School, 1–4–54 Asahimachi, Abeno-ku, Osaka, 545 JapanJP
  2. 2.Third Department of Internal Medicine, Osaka City University Medical School, 1–5–7 Asahimachi, Abeno-ku, Osaka, 545 JapanJP

Personalised recommendations