Cell and Tissue Research

, Volume 303, Issue 1, pp 109–115

Synthesis and localization of the mucin-associated TFF-peptides in the human uterus

  • Antje Wiede
  • Margitta Hinz
  • Eberhard Canzler
  • Kornelia Franke
  • Claudia Quednow
  • Werner Hoffmann
Regular article

DOI: 10.1007/s004410000297

Cite this article as:
Wiede, A., Hinz, M., Canzler, E. et al. Cell Tissue Res (2001) 303: 109. doi:10.1007/s004410000297

Abstract.

TFF-peptides (formerly P-domain peptides, trefoil factors) are typical secretory products of mucin-producing cells and are thought to influence the rheological properties of mucous gels. Here, the localization of these peptides in the human uterus was investigated. An analysis of TFF-peptides mRNA by the polymerase chain reaction revealed TFF3 mainly in the endocervix and smaller amounts in the endometrium. TFF1 and TFF2 mRNA was detectable occasionally in the endocervix and very rarely in the endometrium. Western blot analysis detected only TFF3 in tissue extracts of the endocervix and as a constituent of human cervical mucus. Immunofluorescence localized TFF3 in the surface epithelium of the endocervix and in gland-like structures of the cervical epithelium.

TFF-domain Uterus Endocervix Endometrium Mucin Human 

Copyright information

© Springer-Verlag 2000

Authors and Affiliations

  • Antje Wiede
    • 1
  • Margitta Hinz
    • 1
  • Eberhard Canzler
    • 2
  • Kornelia Franke
    • 3
  • Claudia Quednow
    • 4
  • Werner Hoffmann
    • 1
  1. 1.Institut für Molekularbiologie und Medizinische Chemie, Universitätsklinikum, Otto-von-Guericke-Universität, Leipziger Str. 44, 39120 Magdeburg, Germany
  2. 2.Klinik für Gynäkologie und Geburtshilfe, Städtisches Klinikum, 39130 Magdeburg, Germany
  3. 3.Universitätsfrauenklinik, Otto-von-Guericke-Universität, 39120 Magdeburg, Germany
  4. 4.Institut für Pathologie, Städtisches Klinikum, 39104 Magdeburg, Germany

Personalised recommendations