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Cell and Tissue Research

, Volume 302, Issue 3, pp 397–400 | Cite as

Localization of retinal dehydrogenase type 1 in the stomach and intestine

  • Andréa Frota-Ruchon
  • Mieczyslaw Marcinkiewicz
  • Pangala V. Bhat
Short Communication

Abstract.

Retinal dehydrogenase type 1 (RALDH1) is involved in the biosynthesis of retinoic acid (RA), a modulator of gene expression and cell differentiation. RALDH1 mRNA transcripts are present in the stomach and small intestine, and their expression is regulated by vitamin A status. In situ hybridization demonstrated RALDH1 mRNA expression in epithelial cells of the stomach, small intestine, and large intestine. Strong hybridization was also seen in the lamina propria of small intestinal mucosa and the smooth muscle layer of the small and large intestines. Immunocytochemical localization revealed RALDH1 staining in parietal cells of the stomach and prismatic cells of the small and large intestines. The presence of RALDH1 protein was also detectable within supportive glial cells around neuronal fibers throughout the muscular layers of the stomach as well as the small and large intestines. These data suggest an important role for RALDH1 in generating RA needed for the differentiation of specific epithelial cells in the stomach and intestines.

In situ hybridization Immunocytochemistry Epithelium Plasmocytes Auerbach plexus Retinoic acid Rat (Sprague Dawley) 

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Copyright information

© Springer-Verlag 2000

Authors and Affiliations

  • Andréa Frota-Ruchon
    • 1
  • Mieczyslaw Marcinkiewicz
    • 1
  • Pangala V. Bhat
    • 2
  1. 1.Laboratory of Biochemical Neuroendocrinology, Institut de Recherches Cliniques de Montréal, Canada
  2. 2.Laboratory of Nutrition and Cancer, Research Centre, CHUM-Hôtel-Dieu, 3850 St. Urbain Street, Montréal, Quebec, Canada H2W 1T8

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