Human orbital adipose tissue-derived mesenchymal stem cells possess neuroectodermal differentiation and repair ability
Mesenchymal stem cells (MSCs) are used extensively in cell therapy for repair and regeneration of several organs and tissues. Cell therapy is a valuable option to treat neurodegenerative diseases and MSCs have been shown to improve neuronal function through direct differentiation or secretion of neurotrophic factors. In the present study, we isolated and characterized stem cells from medial and central orbital adipose tissue and found that they could be grown in a monolayer culture. The orbital adipose tissue-derived cells were identical to bone marrow-derived MSCs in their cell surface marker expression, gene expression and multilineage differentiation abilities. The orbital adipose-derived MSCs (OAMSCs) express several neurotrophic factors, possess neuroectodermal differentiation ability and secreted factors from OAMSCs abrogated neuronal cell damage induced by oxidative stress. Thus, OAMSCs might be a valuable cell source for treatment of neurological diseases and to reverse oxidative damage in the neuronal cells.
KeywordsStem cells Neuroprotection Cell therapy Neurodegenerative disease Oxidative damage
DM, AS, RS and AK were supported by MHRD, Govt. of India.
KB and BGJ conceived the study. DM, AK and BGJ designed the study. KB performed blepharoplasty surgery. DM, AS, RS and AK performed experiments. DM, KB, JB, HB, ND and BGJ analyzed the data. DM, KB and BGJ wrote the manuscript. All the authors approved the final version of the manuscript.
This work was supported by funds from the Indian Institute of Technology Guwahati (IITG).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
All procedures involving human samples were in accordance with the Helsinki convention and approved by the ethics committee of the Indian Institute of Technology Guwahati and Sri Sankaradeva Nethralaya, Guwahati. The study was carried out in accordance with the human ethics committee guidelines and written informed consent was obtained from all the patients involved in the study.
- Hu BY, Weick JP, Yu JY, Ma LX, Zhang XQ, Thomson JA, Zhang SC (2010) Neural differentiation of human induced pluripotent stem cells follows developmental principles but with variable potency. Proc Natl Acad Sci U S A 107:4335–4340. https://doi.org/10.1073/pnas.0910012107 CrossRefPubMedPubMedCentralGoogle Scholar
- Maisel M et al (2010) Genome-wide expression profiling and functional network analysis upon neuroectodermal conversion of human mesenchymal stem cells suggest HIF-1 and miR-124a as important regulators. Exp Cell Res 316:2760–2778. https://doi.org/10.1016/j.yexcr.2010.06.012 CrossRefPubMedGoogle Scholar
- PolazziE, MengoniI, Pena-AltamiraE, MassenzioF, VirgiliM, PetrallaS, MontiB (2015) Neuronal regulation of neuroprotective microglial apolipoprotein E secretion in rat in vitro models of brain pathophysiology. J Neuropathol Exp Neurol 74:818–834. https://doi.org/10.1097/Nen.0000000000000222 CrossRefGoogle Scholar
- Togarrati PP et al (2017) Identification and characterization of a rich population of CD34(+) mesenchymal stem/stromal cells in human parotid, sublingual and submandibular glands. Sci Rep 7:3484z. https://doi.org/10.1038/S41598-017-03681-1