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Sialoglycoprotein isolated from Carassius auratus eggs promotes osteogenesis by stimulating mesenchymal stem cells to commit to osteoblast differentiation

  • Lei Mao
  • Meiling Wang
  • Xingjun Xi
  • Yufeng Dai
  • Na Wang
  • Jingfeng WangEmail author
  • Changhu Xue
Regular Article
  • 25 Downloads

Abstract

In this study, we explore whether the pro-osteogenic effects of sialoglycoprotein from Carassius auratus eggs (Ca-SGP) involve mesenchymal stem cells (MSCs). Ovariectomized osteoporotic mice treated with Ca-SGP had increased bone formation and reduced bone marrow adipose tissue. As MSCs are common progenitors of osteoblasts and adipocytes, we isolated MSCs from Ca-SGP-treated mice and found that they tended to differentiate into osteoblasts over adipocytes confirmed by Alizarin red and Oil red O staining. This change was seen at the gene and protein level. To further explore the effect of Ca-SGP on MSCs, we isolated MSCs from healthy mice and treated them with Ca-SGP in vitro. We discovered that Ca-SGP promoted MSC differentiation to osteoblasts. In addition, Ca-SGP promoted osteogenesis and reduced the fat in marrow cavity of adolescent mice. For the first time, our results demonstrate that Ca-SGP promotes osteogenesis via stimulating MSCs to commit to osteoblasts.

Graphical Abstract

Keywords

Sialoglycoprotein Osteogenesis Mesenchymal stem cell Osteoblast Differentiation 

Notes

Funding information

This study is financially supported by the National Key R&D Program of China (No. 2018YFC0311203), National Natural Science Foundation of China (No. 31371876), Key Research & Development Plan of Shandong Province (No. 2016YYSP017) and National Key Research and Development Program (No. 2017YFF0207800).

Compliance with ethical standards

Animal protocols were approved by the ethical committee of experimental animal care at Ocean University of China (certificate no. SYXK20120014). All animal experiments were carried out following institutional guidelines.

Conflicts of interest

The authors declare that they have no competing interest.

Supplementary material

441_2018_2976_MOESM1_ESM.doc (17 kb)
Table S1 Primers used for determination of mRNA expression. (DOC 16 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Lei Mao
    • 1
  • Meiling Wang
    • 1
  • Xingjun Xi
    • 2
  • Yufeng Dai
    • 1
  • Na Wang
    • 1
  • Jingfeng Wang
    • 1
    Email author
  • Changhu Xue
    • 1
  1. 1.College of Food Science and EngineeringOcean University of ChinaQingdaoChina
  2. 2.China National Institute of StandardizationBeijingChina

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