Identification of calretinin-expressing retinal ganglion cells projecting to the mouse superior colliculus
- 206 Downloads
In mice, retinal ganglion cells (RGCs), which consist of around 30 subtypes, exclusively transmit retinal information to the relevant brain systems through parallel visual pathways. The superior colliculus (SC) receives the vast majority of this information from several RGC subtypes. The objective of the current study is to identify the types of calretinin (CR)-expressing RGCs that project to the SC in mice. To label RGCs, we performed CR immunoreactivity in the mouse retina after injections of fluorescent dye, dextran into mouse SC. Subsequently, the neurons double-labeled for dextran and CR were iontophoretically injected with the lipophilic dye, DiI, to characterize the detailed morphological properties of these cells. The analysis of various morphological parameters, including dendritic arborization, dendritic field size and stratification, indicated that, of the ten different types of CR-expressing RGCs in the retina, the double-labeled cells consisted of at least eight types of RGCs that projected to the SC. These cells tended to have small-medium field sizes. However, except for dendritic field size, the cells did not exhibit consistent characteristics for the other morphometric parameters examined. The combination of a tracer and single-cell injections after immunohistochemistry for a particular molecule provided valuable data that confirmed the presence of distinct subtypes of RGCs within multiple-labeled RGCs that projected to specific brain regions.
KeywordsCalretinin Retinal ganglion cells Retrograde tracer injection Single-cell injection Superior colliculus
We thank Cactus Communications for proofreading the manuscript.
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education (NRF-2016R1D1A1A09918427).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All applicable international, national and/or institutional guidelines for the care and use of animals were followed. All procedures involving animals were in accordance with the ethical standards of our institution and were approved by the animal rights committee at Kyungpook National University, Deagu, South Korea (permission NO. 2015-0104). This article does not contain any studies with human participants performed by any of the authors.
- Hof PR, Young WG, Bloom F (2000) Comparative cytoarchitectonic atlas of the C57BL/6 and 129/SV: mouse brains. Elsevier Science, New YorkGoogle Scholar
- Jonathan W, Hiroshi H (2017) Visual system architecture. In: Pablo A (ed) Handbook of visual optics, volume one: fundamentals and eye optics. Fundamentals. CRC Press, Boca Raton, pp 159–180Google Scholar
- Kim T, Soto F, Kerschensteiner D (2015) An excitatory amacrine cell detects object motion and provides feature-selective input to ganglion cells in the mouse retina. elife. https://doi.org/10.7554/eLife.08025
- Rodieck RW (1998) The first steps in seeing. Sinauer Associates, SunderlandGoogle Scholar
- Roska B, Meister M (2014) The retina dissects the visual scene in distinct features. In: Werner JS, Chalupa LM (eds) The new visual neuroscience, retinal mechanisms and processes. MIT Press, Cambridge, pp 163–182Google Scholar
- Schmolesky M (1995-2005) The primary visual cortex. In: Kolb H, Fernandez E, Nelson R (eds) Webvision, The Organization of the Retina and Visual System (Internet). University of Utah Health Sciences Center, Salt Lake CityGoogle Scholar
- Schwaller B (2014) Calretinin: from a “simple” Ca(2+) buffer to a multifunctional protein implicated in many biological processes. Front Neuroanat. eCollection 2014. https://doi.org/10.3389/fnana.2014.00003