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Relationships of endocrine cells to each other and to other cell types in the human gastric fundus and corpus

  • Josiane Fakhry
  • Martin J. Stebbing
  • Billie Hunne
  • Yulia Bayguinov
  • Sean M. Ward
  • Kent C. Sasse
  • Brid Callaghan
  • Rachel M. McQuade
  • John B. FurnessEmail author
Regular Article
  • 211 Downloads

Abstract

Gastric endocrine cell hormones contribute to the control of the stomach and to signalling to the brain. In other gut regions, enteroendocrine cells (EECs) exhibit extensive patterns of colocalisation of hormones. In the current study, we characterise EECs in the human gastric fundus and corpus. We utilise immunohistochemistry to investigate EECs with antibodies to ghrelin, serotonin (5-HT), somatostatin, peptide YY (PYY), glucagon-like peptide 1, calbindin, gastrin and pancreastatin, the latter as a marker of enterochromaffin-like (ECL) cells. EECs were mainly located in regions of the gastric glands populated by parietal cells. Gastrin cells were absent and PYY cells were very rare. Except for about 25% of 5-HT cells being a subpopulation of ECL cells marked by pancreastatin, colocalisation of hormones in gastric EECs was infrequent. Ghrelin cells were distributed throughout the fundus and corpus; most were basally located in the glands, often very close to parietal cells and were closed cells i.e., not in contact with the lumen. A small proportion had long processes located close to the base of the mucosal epithelium. The 5-HT cells were of at least three types: small, round, closed cells; cells with multiple, often very long, processes; and a subgroup of ECL cells. Processes were in contact with their surrounding cells, including parietal cells. Mast cells had very weak or no 5-HT immunoreactivity. Somatostatin cells were a closed type with long processes. In conclusion, four major chemically defined EEC types occurred in the human oxyntic mucosa. Within each group were cells with distinct morphologies and relationships to other mucosal cells.

Keywords

Oxyntic gland Gastrointestinal hormones Ghrelin 5-Hydroxytryptamine Somatostatin Pancreastatin 

Notes

Funding information

This work was supported by NIH (SPARC) grant ID no. OT2OD023847 (PI Terry Powley) to JBF. SMW was supported by NIH DK57236.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Josiane Fakhry
    • 1
  • Martin J. Stebbing
    • 1
    • 2
  • Billie Hunne
    • 1
  • Yulia Bayguinov
    • 3
  • Sean M. Ward
    • 3
  • Kent C. Sasse
    • 4
    • 5
  • Brid Callaghan
    • 1
  • Rachel M. McQuade
    • 1
    • 2
  • John B. Furness
    • 1
    • 2
    Email author
  1. 1.Department of Anatomy and NeuroscienceUniversity of MelbourneParkvilleAustralia
  2. 2.Florey Institute of Neuroscience and Mental HealthParkvilleAustralia
  3. 3.Department of Physiology and Cell Biology, Reno School of MedicineUniversity of NevadaRenoUSA
  4. 4.School of Medicine, Universiity of NevadaRenoUSA
  5. 5.Renown Regional Medical CenterRenoUSA

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