ATP-binding cassette (ABC) transporters in caprine preantral follicles: gene and protein expression
The multidrug resistance proteins ABCB1, ABCC2 and ABCG2 are an energy-dependent efflux pump that functions in systemic detoxification processes. Physiologically expressed in a variety of tissues, most abundantly in the liver and intestinal epithelia, placenta, blood-brain barrier and various stem cells, until now, these pumps were not identified in goat ovarian tissue. Therefore, the aim of this study is to analyze ABCB1, ABCC2, and ABCG2 mRNA and protein expression in goat preantral follicles. Fragments (3 × 3 × 1 mm) from five pairs of ovary (n = 10) obtained from five goat were collected and immediately submitted to qPCR, Western blot, and immunofluorescence assay for mRNA detection and identification and localization of the ABC transporters, respectively. mRNA for ABCB1, ABCC2, and ABCG2 and the presence of their proteins were observed on ovarian tissue samples. Positive marks were observed for the three transport proteins in all follicular categories studied. However, the marks were primarily localized in the oocyte of primordial, transition and primary follicle categories. In conclusion, goat ovarian tissue expresses mRNA for the ABCB1, ABCC2 and ABCG2 transporters and the expression of these proteins in the preantral follicles is a follicle-dependent stage.
KeywordsABCB1 ABCC2 ABCG2 Preantral follicles Multidrug resistance
The authors would like to thank the National Council for Scientific and Technological Development (CNPq Brazil) for financial support. Denise Damasceno Guerreiro is a recipient of a doctoral scholarship from Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico - (FUNCAP, Brazil). Ana Paula Ribeiro Rodrigues is a recipient of a grant from CNPq Brazil through the projects 473968/2013-4 and 457226/2013-7. Johan Smitz is a Special Visitor Researcher from CAPES (88881.030.433/2013-01).
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