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Myogenic potential of mesenchymal stem cells isolated from porcine adipose tissue

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Abstract

Advances in stem cell biology and materials science have provided a basis for developing tissue engineering methods to repair muscle injury. Among stem cell populations with potential to aid muscle repair, adipose-derived mesenchymal stem cells (ASC) hold great promise. To evaluate the possibility of using porcine ASC for muscle regeneration studies, we co-cultured porcine ASC with murine C2C12 myoblasts. These experiments demonstrated that porcine ASC display significant myogenic potential. Co-culture of ASC expressing green fluorescent protein (GFP) with C2C12 cells resulted in GFP+ myotube formation, indicating fusion of ASC with myoblasts to form myotubes. The presence of porcine lamin A/C positive nuclei in myotubes and RTqPCR analysis of porcine myogenin and desmin expression confirmed that myotube nuclei derived from ASC contribute to muscle gene expression. Co-culturing GFP+ASC with porcine satellite cells demonstrated enhanced myogenic capability of ASC, as the percentage of labeled myotubes increased compared to mouse co-cultures. Enhancing myogenic potential of ASC through soluble factor treatment or expansion of ASC with innate myogenic capacity should allow for their therapeutic use to regenerate muscle tissue lost to disease or injury.

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Acknowledgements

The authors would like to thank Dr. Stephen Kaufman (University of Illinois) for his generous gift of polyclonal α7 integrin antibody, the NIH National Swine Resource and Research Center (NSRRC, University of Missouri-Columbia) for providing the GFP adipose tissue for the GFP ASC isolation and Kathryn Polkoff (University of Illinois) for help with editing of the manuscript. A portion of the work presented here was partially supported by the Carle Foundation Hospital (#2007–04072), Urbana, IL and the Illinois Regenerative Medicine Institute (IDPH Grant # 63080017).

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  1. Carl R. Woese Institute of Genomic Biology, University of Illinois at Urbana-Champaign, 1207 West Gregory Drive, Urbana, IL, 61801, USA

    Derek J. Milner, Massimo Bionaz, Elisa Monaco, Jo Ann Cameron & Matthew B. Wheeler

  2. Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA

    Derek J. Milner, Massimo Bionaz, Elisa Monaco & Matthew B. Wheeler

  3. Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA

    Jo Ann Cameron

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  1. Derek J. Milner
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Milner, D.J., Bionaz, M., Monaco, E. et al. Myogenic potential of mesenchymal stem cells isolated from porcine adipose tissue. Cell Tissue Res 372, 507–522 (2018). https://doi.org/10.1007/s00441-017-2764-z

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