Cell and Tissue Research

, Volume 368, Issue 2, pp 405–410 | Cite as

Dynamic zonation of liver polyploidy

  • Sivan Tanami
  • Shani Ben-Moshe
  • Anat Elkayam
  • Avi Mayo
  • Keren Bahar Halpern
  • Shalev Itzkovitz
Short Communication

Abstract

The liver is a polyploid organ, consisting of hepatocytes with one or two nuclei each containing 2, 4, 8 or more haploid chromosome sets. The dynamic changes in the spatial distributions of polyploid classes across the liver lobule, its repeating anatomical unit, have not been characterized. Identifying these spatial patterns is important for understanding liver homeostatic and regenerative turnover, as well as potential division of labor among ploidy classes. Here, we use single molecule-based tissue imaging to reconstruct the spatial zonation profiles of liver polyploid classes in mice of different ages. We find that liver polyploidy proceeds in spatial waves, advancing more rapidly in the mid-lobule zone compared to the periportal and perivenous zones. We also measure the spatial zonation profiles of S-phase entry at different ages and identify more rapid S-phase entry in the mid-lobule zone at older ages. Our findings reveal fundamental features of liver spatial heterogeneity and highlight their dynamic changes during development and aging.

Keywords

Liver zonation Liver polyploidy Systems biology Single molecule imaging Stem cells 

Supplementary material

441_2016_2427_MOESM1_ESM.pdf (62 kb)
ESM 1(PDF 61 kb)
441_2016_2427_MOESM2_ESM.pdf (53 kb)
ESM 2(PDF 52 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Sivan Tanami
    • 1
  • Shani Ben-Moshe
    • 1
  • Anat Elkayam
    • 1
  • Avi Mayo
    • 1
  • Keren Bahar Halpern
    • 1
  • Shalev Itzkovitz
    • 1
  1. 1.Department of Molecular Cell BiologyWeizmann Institute of ScienceRehovotIsrael

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