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Cell and Tissue Research

, Volume 364, Issue 3, pp 543–557 | Cite as

Postnatal development of the bronchiolar club cells of distal airways in the mouse lung: stereological and molecular biological studies

  • Srikanth Karnati
  • Tilman Graulich
  • Gani Oruqaj
  • Susanne Pfreimer
  • Michael Seimetz
  • Cordula Stamme
  • Thomas J. Mariani
  • Norbert Weissmann
  • Christian Mühlfeld
  • Eveline Baumgart-Vogt
Regular Article

Abstract

Club (Clara) cells are nonciliated secretory epithelial cells present in bronchioles of distal pulmonary airways. So far, no information is available on the postnatal differentiation of club cells by a combination of molecular biological, biochemical, and stereological approaches in the murine lung. Therefore, the present study was designed to investigate the changes in the club cell secretory proteins (CC10, surfactant proteins A, B and D) and club cell abundance within the epithelium of bronchioles of distal airways during the postnatal development of the mouse lung. Perfusion-fixed murine lungs of three developmental stages (newborn, 15-day-old and adult) were used. Frozen, unfixed lungs were used for cryosectioning and subsequent laser-assisted microdissection of bronchiolar epithelial cells and RT-PCR analyses. High resolution analyses of the three-dimensional structures and composition of lung airways were obtained by scanning electron microscopy. Finally, using design-based stereology, the total and average club cell volume and the volume of secretory granules were quantified by light and transmission electron microscopy. Our results reveal that murine club cells are immature at birth and differentiate postnatally. Further, increase of the club cell volume and number of intracellular granules are closely correlated to the total lung volume enlargement. However, secretory granule density was only increased within the first 15 days of postnatal development. The differentiation is accompanied by a decrease in glycogen content, and a close positive relationship between CC10 expression and secretory granule abundance. Taken together, our data are consistent with the concept that the morphological and functional differentiation of club cells is a postnatal phenomenon.

Keywords

Clara CC10 Stereology Lung Electron microscopy Surfactant proteins BASCs 

Notes

Acknowledgments

The excellent technical assistance of Bianca Pfeiffer, Gerd Magdowski, Gerhard Kripp, Ulrich Gärtner, Anika Seipp and Karina Greve is gratefully acknowledged. Further, we would like to thank Barry Stripp (Departments of Medicine and Cell Biology, Duke University Medical Center, Durham, North Carolina) for providing CC10 wild-type and knockout tissue samples. Our study was supported by LOM (Leistungsorientierte Mittel) performance-related resource allocation-funds of the Medical Faculty of the Justus Liebig University Giessen, and by the DFG via the Cluster of Excellence REBIRTH, Germany.

Supplementary material

441_2015_2354_MOESM1_ESM.jpg (387 kb)
Fig. 1 Immunofluorescence detection of BASCs during the postnatal development of the mouse lung. Paraffin-embedded mouse tissue sections of newborn, 15-day-old and adult lungs were stained with CC10 and pro SP-C (A-C). Respective higher magnifications of these stainings are depicted in D-F. BASCs are located at the branching point between a terminal bronchiole lined with club cells positive for CC10 and the alveolar space lined with the alveolar epithelium including AECII cells positive for pro SP-C. Note that BASCs were increased during postnatal development of the mouse lung. *erythrocytes; Bars (a–c) 50 μm, d–f, j–l) 20 μm. (JPG 386 kb).

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Srikanth Karnati
    • 1
  • Tilman Graulich
    • 2
  • Gani Oruqaj
    • 1
  • Susanne Pfreimer
    • 1
  • Michael Seimetz
    • 3
  • Cordula Stamme
    • 4
  • Thomas J. Mariani
    • 5
  • Norbert Weissmann
    • 3
  • Christian Mühlfeld
    • 6
    • 7
  • Eveline Baumgart-Vogt
    • 1
  1. 1.Institute for Anatomy and Cell Biology II, Division of Medical Cell BiologyJustus Liebig UniversityGiessenGermany
  2. 2.Department of TraumaHannover Medical SchoolHannoverGermany
  3. 3.Excellence Cluster Cardio-Pulmonary System (ECCPS), Member of the German Center for Lung Research (DZL), Universities of Giessen and Marburg Lung Center (UGMLC)Justus Liebig University GiessenGiessenGermany
  4. 4.Division of Cellular Pneumology, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Borstel, Germany and Department of AnesthesiologyUniversity of LübeckLübeckGermany
  5. 5.Division of Neonatology and Pediatric Molecular and Personalized Medicine ProgramUniversity of Rochester Medical CenterRochesterUSA
  6. 6.Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH)Member of the German Center for Lung Research (DZL)HannoverGermany
  7. 7.Institute of Functional and Applied AnatomyHannover Medical SchoolHannoverGermany

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