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Cell and Tissue Research

, Volume 363, Issue 2, pp 541–554 | Cite as

Transient up- and down-regulation of expression of myosin light chain 2 and myostatin mRNA mark the changes from stratified hyperplasia to muscle fiber hypertrophy in larvae of gilthead sea bream (Sparus aurata L.)

  • Stella Georgiou
  • Hélène Alami-Durante
  • Deborah M. Power
  • Elena Sarropoulou
  • Zissis Mamuris
  • Katerina A. MoutouEmail author
Regular Article

Abstract

Hyperplasia and hypertrophy are the two mechanisms by which muscle develops and grows. We study these two mechanisms, during the early development of white muscle in Sparus aurata, by means of histology and the expression of structural and regulatory genes. A clear stage of stratified hyperplasia was identified early in the development of gilthead sea bream but ceased by 35 dph when hypertrophy took over. Mosaic recruitment of new white fibers began as soon as 60 dph. The genes mlc2a and mlc2b were expressed at various levels during the main phases of hyperplasia and hypertrophy. The genes myog and mlc2a were significantly up-regulated during the intensive stratified formation of new fibers and their expression was significantly correlated. Expression of mstn1 and igf1 increased at 35 dph, appeared to regulate the hyperplasia-to-hypertrophy transition, and may have stimulated the expression of mlc2a, mlc2b and col1a1 at the onset of mosaic hyperplasia. The up-regulation of mstn1 at transitional phases in muscle development indicates a dual regulatory role of myostatin in fish larval muscle growth.

Keywords

Gene markers mlc2 Muscle cellularity Myogenic factors Gilthead sea bream 

Notes

Acknowledgments

The authors thank M. Cluzeaud and D. Bazin for their technical support.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Stella Georgiou
    • 1
  • Hélène Alami-Durante
    • 2
  • Deborah M. Power
    • 3
  • Elena Sarropoulou
    • 4
  • Zissis Mamuris
    • 1
  • Katerina A. Moutou
    • 1
    Email author
  1. 1.Department of Biochemistry & BiotechnologyUniversity of ThessalyLarissaGreece
  2. 2.UR 1067 Nutrition Métabolisme Aquaculture, INRA, AquapôleSaint-Pée-sur-NivelleFrance
  3. 3.Centre of Marine Sciences (CCMar)Universidade do AlgarveFaroPortugal
  4. 4.Institute of Marine Biology & GeneticsHellenic Centre for Marine ResearchHeraklionGreece

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