Cell and Tissue Research

, Volume 362, Issue 3, pp 513–527 | Cite as

Molecular organization and fine structure of the human tectorial membrane: is it replenished?

  • Hisamitsu Hayashi
  • Annelies Schrott-Fischer
  • Rudolf Glueckert
  • Wei Liu
  • Willi Salvenmoser
  • Peter Santi
  • Helge Rask-Andersen
Regular Article

Abstract

Auditory sensitivity and frequency resolution depend on the physical properties of the basilar membrane in combination with outer hair cell-based amplification in the cochlea. The physiological role of the tectorial membrane (TM) in hair cell transduction has been controversial for decades. New insights into the TM structure and function have been gained from studies of targeted gene disruption. Several missense mutations in genes regulating the human TM structure have been described with phenotypic expressions. Here, we portray the remarkable gradient structure and molecular organization of the human TM. Ultrastructural analysis and confocal immunohistochemistry were performed in freshly fixed human cochleae obtained during surgery. Based on these findings and recent literature, we discuss the role of human TMs in hair cell activation. Moreover, the outcome proposes that the α-tectorin-positive amorphous layer of the human TM is replenished and partly undergoes regeneration during life.

Keywords

Tectorial membrane Human Electron microscopy α-tectorin 

Abbreviation

BM

Basilar membrane

HS

Hensen’s stripe

IDC

Interdental cell

IHC

Inner hair cell

KM

Kimura’s membrane

L

Limbus

LZ

Limbal zone

MaZ

Marginal zone

MN

Marginal net

MZ

Middle zone

OHC

Outer hair cell

RF

Radial fiber

SEM

Scanning electron microscopy

STL

Subtectorial layer

TEM

Transmission electron microscopy

TM

Tectorial membrane

Notes

Acknowledgments

This study was supported by ALF grants from Uppsala University Hospital and Uppsala University and by the Tysta Skolan Foundation, Swedish Deafness Foundation (HRF) and Land Tirol Technologie Förderungsprogram, Förderung von Wissenschaft, Forschung und Entwicklung (Programm K-Regio Vamel) and Med El, Innsbruck, Austria. Our research is part of the European Community 7th Framework Programme on Research, Technological Development and Demonstration. Project acronym: NANOCI. Grant agreement no: 281056. It was also supported by kindly donated private funds from Börje Runögård, Sweden. Dr. Klaus Qvortrup is acknowledged for providing oxygenated fluorocarbon fixative for the TEM investigation.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Hisamitsu Hayashi
    • 1
    • 2
  • Annelies Schrott-Fischer
    • 3
  • Rudolf Glueckert
    • 3
  • Wei Liu
    • 4
  • Willi Salvenmoser
    • 5
  • Peter Santi
    • 6
  • Helge Rask-Andersen
    • 1
  1. 1.Department of Surgical Sciences, Head and Neck Surgery, section of OtolaryngologyUppsala University HospitalUppsalaSweden
  2. 2.Department of OtolaryngologyGifu University Graduate School of MedicineGifuJapan
  3. 3.Department of OtolaryngologyMedical University of InnsbruckInnsbruckAustria
  4. 4.Department of Surgical Sciences, Section of OtolaryngologyUppsala University HospitalUppsalaSweden
  5. 5.Institute of ZoologyUniversity of InnsbruckInnsbruckAustria
  6. 6.Department of OtolaryngologyUniversity of MinnesotaMinneapolisUSA

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