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Cell and Tissue Research

, Volume 362, Issue 2, pp 367–377 | Cite as

Comparative analysis of neural differentiation potential in human mesenchymal stem cells derived from chorion and adult bone marrow

  • Reihane Ziadlou
  • Maryam ShahhoseiniEmail author
  • Fatemeh Safari
  • Forugh-Azam Sayahpour
  • Shiva Nemati
  • Mohamadreza Baghaban EslaminejadEmail author
Regular Article

Abstract

The finding of a reliable and abundant source of stem cells for the replacement of missing neurons in nervous system diseases requires extensive characterization of neural-differentiation-associated markers in stem cells from various sources. Chorion-derived stem cells from the human placenta have recently been described as an abundant, ethically acceptable, and easily accessible source of cells that are not limited in the same way as bone marrow (BM) mesenchymal stem cells (MSCs). We have isolated and cultured chorion MSCs (C-MSCs) and compared their proliferative capacity, multipotency, and neural differentiation ability with BM-MSCs. C-MSCs showed a higher proliferative capacity compared with BM-MSCs. The expression and histone modification of Nestin, as a marker for neural stem/progenitor cells, was evaluated quantitatively between the two groups. The Nestin expression level in C-MSCs was significantly higher than that in BM-MSCs. Notably, modifications of lys9, lys4, and lys27 of histone H3 agreed with the remarkable higher expression of Nestin in C-MSCs than in BM-MSCs. Furthermore, after neural differentiation of MSCs upon retinoic acid induction, both immunocytochemical and flow cytometry analyses demonstrated that the expression of neural marker genes was significantly higher in neural-induced C-MSCs compared with BM-MSCs. Mature neuron marker genes were also expressed at a significantly higher level in C-MSCs than in BM-MSCs. Thus, C-MSCs have a greater potential than BM-MSCs for differentiation to neural cell lineages and can be regarded as a promising source of stem cells for the cell therapy of neurological disorders.

Keywords

Chorion Bone marrow Mesenchymal stem cells Neural differentiation Human 

Notes

Acknowledgments

We thank Raha Favaedi for her kind technical support of the project.

Supplementary material

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Fig. S1

(GIF 50 kb)

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High resolution image (TIFF 483 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Reihane Ziadlou
    • 1
    • 2
    • 3
  • Maryam Shahhoseini
    • 1
    Email author
  • Fatemeh Safari
    • 2
  • Forugh-Azam Sayahpour
    • 2
  • Shiva Nemati
    • 2
  • Mohamadreza Baghaban Eslaminejad
    • 2
    Email author
  1. 1.Department of Genetics at Reproductive Biomedicine Research CenterRoyan Institute for Reproductive Biomedicine, ACECRTehranIran
  2. 2.Department of Stem Cells and Developmental Biology at Cell Science Research CenterRoyan Institute for Stem Cell Biology and Technology, ACECRTehranIran
  3. 3.Department of Molecular and Cellular Biology, Faculty of Basic Sciences and Advanced Technologies in BiologyUniversity of Science and CultureTehranIran

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