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Cell and Tissue Research

, Volume 361, Issue 3, pp 745–753 | Cite as

Three-dimensional differentiation of adipose-derived mesenchymal stem cells into insulin-producing cells

  • Layasadat KhorsandiEmail author
  • Ali Khodadadi
  • Fereshteh Nejad-Dehbashi
  • Sadegh Saremy
Regular Article

Abstract

The aim of this study is to evaluate the collagen/hyaluronic acid (Col/HA) scaffold effect on the differentiation of insulin-producing cells (IPCs) from adipose-derived mesenchymal stem cells (ASCs). In this experimental study, ASCs were cultured and seeded in a Col/HA scaffold (3D culture) and then treated with induction media. After induction, the presence of IPCs was evaluated using gene expression (PDX-1, GLUT-2 and insulin) analysis and immunocytochemistry, while functional maturity was determined by measuring insulin release in response to low- and high-glucose media. The induced IPCs were morphologically similar to pancreatic islet-like cells. Expression of the islet-associated genes PDX-1, GLUT-2 and insulin genes in 3D-cultured cells was markedly higher than the 2D-cultured cells exposure differentiation media. Compared to the 2D culture of ASCs-derived IPCs, the insulin release from 3D ASCs-derived IPCs showed a nearly 4-fold (p < 0.05) increase when exposed to a high glucose (25 mmol) medium. The percentage of insulin-positive cells in the 3D experimental group showed an approximately 4-fold increase compared to the 2D experimental culture cells. The results of this study demonstrated that the COL/HA scaffold can enhance the differentiation of IPCs from rat ASCs.

Keywords

Collagen/hyaluronic acid scaffold Adult stem cells PDX-1 GLUT-2 Insulin 

Notes

Acknowledgment

The financial cost of this project has been provided by the research council of the Ahvaz Jundishapur University of Medical Sciences (Grant number: CMRC-48).

Supplementary material

441_2015_2140_MOESM1_ESM.xml (9 kb)
ESM 1 (XML 9 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Layasadat Khorsandi
    • 1
    • 2
    Email author
  • Ali Khodadadi
    • 1
    • 3
  • Fereshteh Nejad-Dehbashi
    • 1
  • Sadegh Saremy
    • 1
  1. 1.Cell & Molecular Research Center, Faculty of MedicineAhvaz Jundishapur University of Medical SciencesAhvazIran
  2. 2.Department of Anatomical Sciences, Faculty of MedicineAhvaz Jundishapur University of Medical SciencesAhvazIran
  3. 3.Department of Immunology and Petroleum and Environmental Pollutants Research Center, Faculty of MedicineJundishapur University of Medical SciencesAhvazIran

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