Cell and Tissue Research

, Volume 360, Issue 1, pp 13–28

Confocal stereology: an efficient tool for measurement of microscopic structures

Review

Abstract

Quantitative measurements of geometric forms or counting of objects in microscopic specimens is an essential tool in studies of microstructure. Confocal stereology represents a contemporary approach to the evaluation of microscopic structures by using a combination of stereological methods and confocal microscopy. 3-D images acquired by confocal microscopy can be used for the estimation of geometrical characteristics of microscopic structures by stereological methods, based on the evaluation of optical sections within a thick slice and using computer-generated virtual test probes. Such methods can be used for estimating volume, number, surface area and length using relevant spatial probes, which are generated by specific software. The interactions of the probes with the structure under study are interactively evaluated. An overview of the methods of confocal stereology developed during the past 30 years is presented. Their advantages and pitfalls in comparison with other methods for measurement of geometrical characteristics of microscopic structures are discussed.

Keywords

3-D images Confocal microscopy Geometrical characteristics Spatial probes Stereology 

Supplementary material

441_2015_2138_MOESM1_ESM.avi (489 kb)
Movie 1(AVI 488 kb)
441_2015_2138_MOESM2_ESM.avi (997 kb)
Movie 2(AVI 997 kb)
441_2015_2138_MOESM3_ESM.avi (994 kb)
Movie 3(AVI 994 kb)
441_2015_2138_MOESM4_ESM.avi (622 kb)
Movie 4(AVI 622 kb)
441_2015_2138_MOESM5_ESM.avi (787 kb)
Movie 5(AVI 787 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Department of Biomathematics, Institute of PhysiologyAcademy of Sciences of the Czech RepublicPragueCzech Republic

Personalised recommendations