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Cell and Tissue Research

, Volume 360, Issue 2, pp 309–319 | Cite as

Oncostatin M regulates osteogenic differentiation of murine adipose-derived mesenchymal progenitor cells through a PKCdelta-dependent mechanism

  • David C. Smyth
  • Shunsuke Takenaka
  • Celine Yeung
  • Carl D. RichardsEmail author
Regular Article

Abstract

Oncostatin M (OSM) is an IL-6/LIF family cytokine that influences mesenchymal progenitor differentiation; however, the mechanisms of this activity have not been fully elucidated. Using uncommitted murine adipose tissue-derived mesenchymal progenitors, we have examined mechanisms of OSM-induced osteogenesis. Murine OSM (mOSM) induced osteogenic differentiation to a greater degree than interleukin (IL)-6 and other members of the gp130 cytokine family, promoting extracellular matrix mineralization as indicated by Alizarin Red S staining. mOSM also increased expression of osteogenesis-associated gene products BMP4, BMP7, Runx-2, and osteocalcin as assessed by immunoblotting and real-time quantitative PCR. Additionally, protein kinase C (PKC) delta activity was upregulated in response to OSM stimulation, and to a greater degree than IL-6. Knockdown of PKCdelta expression by use of RNA interference (RNAi) reduced OSM-mediated osteogenic differentiation and decreased expression of Runx-2. These findings suggest that OSM differentially promotes osteogenesis in non-committed mesenchymal progenitors relative to other gp130 cytokines. This activity correlates with selective activation of PKCdelta in OSM-treated cells, indicating that OSM-induced osteogenesis and upregulation of osteogenic gene products require activity of PKCdelta.

Keywords

Osteogenesis Signal transduction Gene regulation Cytokines Oncostatin M 

Notes

Acknowledgments

The authors want to thank Christine Demers, Carrie M. Langdon, and Rebecca Rodrigues (McMaster University) for excellent technical assistance, and Steven Wong (McMaster University) for review of the manuscript. This work was supported by CIHR grant # 102565.

Conflict of Interest

None declared.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • David C. Smyth
    • 1
  • Shunsuke Takenaka
    • 2
  • Celine Yeung
    • 2
  • Carl D. Richards
    • 2
    • 3
    Email author
  1. 1.Currently at University of Ottawa Heart InstituteUniversity of OttawaOttawaCanada
  2. 2.McMaster Immunology Research Centre, Department of Pathology and Molecular MedicineMcMaster UniversityHamiltonCanada
  3. 3.MDCL-4017, Department of Pathology and Molecular MedicineMcMaster UniversityHamiltonCanada

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