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Cell and Tissue Research

, Volume 360, Issue 2, pp 223–231 | Cite as

Acid-sensing ion channels (ASICs) 2 and 4.2 are expressed in the retina of the adult zebrafish

  • E. Viña
  • V. Parisi
  • C. Sánchez-Ramos
  • R. Cabo
  • M. C. Guerrera
  • L. M. Quirós
  • A. Germanà
  • J. A. VegaEmail author
  • O. García-Suárez
Regular Article

Abstract

Acid-sensing ion channels (ASICs) are H+-gated, voltage-insensitive cation channels involved in synaptic transmission, mechanosensation and nociception. Different ASICs have been detected in the retina of mammals but it is not known whether they are expressed in adult zebrafish, a commonly used animal model to study the retina in both normal and pathological conditions. We study the expression and distribution of ASIC2 and ASIC4 in the retina of adult zebrafish and its regulation by light using PCR, in situ hybridization, western blot and immunohistochemistry. We detected mRNA encoding zASIC2 and zASIC4.2 but not zASIC4.1. ASIC2, at the mRNA or protein level, was detected in the outer nuclear layer, the outer plexiform layer, the inner plexiform layer, the retinal ganglion cell layer and the optic nerve. ASIC4 was expressed in the photoreceptors layer and to a lesser extent in the retinal ganglion cell layer. Furthermore, the expression of both ASIC2 and ASIC4.2 was down-regulated by light and darkness. These results are the first demonstration that ASIC2 and ASIC4 are expressed in the adult zebrafish retina and suggest that zebrafish could be used as a model organism for studying retinal pathologies involving ASICs.

Keywords

Retina Acid-sensing ion channels Light Zebrafish 

Notes

Acknowledgment

The antibody against ASIC4.2 zebrafish was kindly provided by Dr. Greg Goss, University of Alberta, Canada.

Supplementary material

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • E. Viña
    • 1
    • 2
  • V. Parisi
    • 3
  • C. Sánchez-Ramos
    • 4
    • 5
  • R. Cabo
    • 1
  • M. C. Guerrera
    • 3
  • L. M. Quirós
    • 6
  • A. Germanà
    • 3
  • J. A. Vega
    • 1
    • 7
    Email author
  • O. García-Suárez
    • 1
  1. 1.Departamento de Morfología y Biología CelularGrupo SINPOS Universidad de OviedoOviedoSpain
  2. 2.Servicio de Cuidados IntensivosHospital de CabueñesGijónSpain
  3. 3.Dipartimento di Scienze VeterinarieUniversità di MessinaMessinaItaly
  4. 4.Departamento de Óptica II (Optometría y Visión), and Grupo de Neurocomputación y NeurorrobóticaUniversidad Complutense de MadridMadridSpain
  5. 5.Grupo de Neurocomputación y NeurorrobóticaUniversidad Complutense de MadridMadridSpain
  6. 6.Departamento de Biología FuncionalUniversidad de OviedoOviedoSpain
  7. 7.Facultad de Ciencias de la SaludUniversidad Autonoma de ChileTemucoChile

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