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Cell and Tissue Research

, Volume 358, Issue 1, pp 149–164 | Cite as

Characterization and evaluation of mesenchymal stem cells derived from human embryonic stem cells and bone marrow

  • Patrick T. Brown
  • Matthew W. Squire
  • Wan-Ju LiEmail author
Regular Article

Abstract

Embryonic stem cells (ESCs) and mesenchymal stem cells (MSCs) have been studied for years as primary cell sources for regenerative biology and medicine. MSCs have been derived from cell and tissue sources, such as bone marrow (BM), and more recently from ESCs. This study investigated MSCs derived from BM, H1- and H9-ESC lines in terms of morphology, surface marker and growth factor receptor expression, proliferative capability, modulation of immune cell growth and multipotency, in order to evaluate ESC-MSCs as a cell source for potential regenerative applications. The results showed that ESC-MSCs exhibited spindle-shaped morphology similar to BM-MSCs but of various sizes, and flow cytometric immunophenotyping revealed expression of characteristic MSC surface markers on all tested cell lines except H9-derived MSCs. Differences in growth factor receptor expression were also shown between cell lines. In addition, ESC-MSCs showed greater capabilities for cell proliferation, and suppression of leukocyte growth compared to BM-MSCs. Using standard protocols, induction of ESC-MSC differentiation along the adipogenic, osteogenic, or chondrogenic lineages was less effective compared to that of BM-MSCs. By adding bone morphogenetic protein 7 (BMP7) into transforming growth factor beta 1 (TGFβ1)-supplemented induction medium, chondrogenesis of ESC-MSCs was significantly enhanced. Our findings suggest that ESC-MSCs and BM-MSCs show differences in their surface marker profiles and the capacities of proliferation, immunomodulation, and most importantly multi-lineage differentiation. Using modified chondrogenic medium with BMP7 and TGFβ1, H1-MSCs can be effectively induced as BM-MSCs for chondrogenesis.

Keywords

Embryonic stem cell Mesenchymal stem cell Differentiation Transforming growth factor beta Bone morphogenetic protein 

Notes

Acknowledgments

Patrick Brown was supported by NIH/NIGMS R25 GM083252 through the TEAM Science Program and the UW SMPH MSTP T32 GM008692. The authors would like to thank Drs. Igor Slukvin, Xin Zhang, Kran Suknuntha, Peiman Hematti and Jaehyup Kim for providing ESC-MSCs and PBMCs for this study.

Disclosure statement

The authors have no financial conflicts to disclose.

Supplementary material

441_2014_1926_MOESM1_ESM.pdf (79 kb)
ESM 1 (PDF 79 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Patrick T. Brown
    • 1
    • 2
  • Matthew W. Squire
    • 1
  • Wan-Ju Li
    • 1
    • 3
    • 4
    Email author
  1. 1.Department of Orthopedics and RehabilitationUniversity of Wisconsin-MadisonMadisonUSA
  2. 2.Graduate Program in Cellular and Molecular BiologyUniversity of Wisconsin-MadisonMadisonUSA
  3. 3.Department of Biomedical EngineeringUniversity of Wisconsin-Madison1550 Engineering DriveUSA
  4. 4.Musculoskeletal Biology and Regenerative Medicine Laboratory, Department of Orthopedics and RehabilitationUniversity of Wisconsin-MadisonMadisonUSA

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