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Cell and Tissue Research

, Volume 357, Issue 3, pp 563–569 | Cite as

Anoctamin 1 expression in the mouse auditory brainstem

  • Sang Jae Cho
  • Ji Hyun Jeon
  • Do Ill Chun
  • Sang Won YeoEmail author
  • In-Beom KimEmail author
Short Communication

Abstract

Calcium-activated chloride channels (CaCCs) are involved in numerous physiological functions, including the epithelial movement of fluid. Anoctamin 1 (ANO1) has recently been cloned and characterized as a CaCC and is known to be expressed in various secretory epithelia and in nervous tissues such as the dorsal root ganglia and retina. However, data regarding the expression, function, and cellular and subcellular localization of CaCCs in the brain are still limited. We investigated the distribution and expression patterns of ANO1 in adult mouse brain. Reverse transcriptase plus the polymerase chain reaction, Western blot, and immunohistochemical analyses demonstrated that ANO1 was widely distributed throughout the brain. Furthermore, ANO1 was strongly expressed in two auditory brainstem nuclei: the medial nucleus of the trapezoid body (MNTB) and the anteroventral cochlear nucleus (AVCN). Double-labeling experiments revealed that this ANO1 expression was exclusive to the presynaptic endings of both the MNTB and AVCN. ANO1 is thus mainly localized at presynaptic terminals in various brain regions, specifically in two auditory brainstem nuclei, the MNTB and AVCN, and might therefore contribute to the high-frequency synaptic transmission of auditory signals.

Keywords

Anoctamin 1 Calcium-activated chloride channel Medial nucleus of the trapezoid body Anteroventral cochlear nucleus Synapse Mouse 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Department of Otolaryngology-Head and Neck Surgery, College of MedicineThe Catholic University of KoreaSeoulKorea
  2. 2.Department of Anatomy, College of MedicineThe Catholic University of KoreaSeoulKorea
  3. 3.2012-2013 Summer Biomedical Research Program for High School Students, College of MedicineThe Catholic University of KoreaSeoulKorea
  4. 4.Catholic Neuroscience Institute, College of MedicineThe Catholic University of KoreaSeoulKorea

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